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Literature summary for 4.3.1.1 extracted from

  • La, I.; Kalme, S.; Yoon, M.
    Reversible denaturation and reactivation of aspartase from Hafnia alvei (2008), Bull. Korean Chem. Soc., 29, 208-210.
No PubMed abstract available
Search for more literature for 4.3.1.1

Application

Application Comment Organism
industry aspartate synthesis Hafnia alvei

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride at concentrations lower than 1 M, activity is gradually decreased suggesting the existence of the native tetrameric form with denaturation intermediates such as dimeric and monomeric forms of the enzyme. At higher concentrations above 1 M, the enzyme completely lost the activity, suggesting that the enzyme structure is completely denatured Hafnia alvei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
 Hafnia alvei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate Hafnia alvei
-
 fumarate + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Hafnia alvei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by using a combination of diethylaminoethyl cellulose, Red A-agarose,and Sepharose 6B chromatography Hafnia alvei

Renatured (Commentary)

Renatured (Comment) Organism
100% activity recovery is observed when the enzyme is renatured by dilution at concentrations below 1 M guanidine hydrochloride, the renaturation yield at concentrations above 1 M is 40%. The dissociation process from native tetramer to dimer is reversible but the dissociation process from dimer to monomer is not reversible. Hafnia alvei

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Hafnia alvei
-

Storage Stability

Storage Stability Organism
the catalytic function of the purified enzyme which stored at -70°C until use remains stable for at least 1 month at 4°C without appreciable loss of enzymatic activity Hafnia alvei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
 Hafnia alvei fumarate + NH3
-
 r

Subunits

Subunits Comment Organism
homotetramer
-
 Hafnia alvei

Synonyms

Synonyms Comment Organism
aspartase
-
 Hafnia alvei
L-aspartate ammonia-lyase
-
 Hafnia alvei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 optimum temperature for reactivation Hafnia alvei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 35 no reactivation between 0-10°C, linear increase at 10-25°C, with a level off between 25-35°C and completely stopped at 35°C Hafnia alvei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 optimum for reactivation Hafnia alvei