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Literature summary for 4.3.1.1 extracted from

  • Kazuoka, T.; Masuda, Y.; Oikawa, T.; Soda, K.
    Thermostable aspartase from a marine psychrophile, Cytophaga sp. KUC-1: molecular characterization and primary structure (2003), J. Biochem., 133, 51-58.
    View publication on PubMed
Search for more literature for 4.3.1.1

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 2 mM, 98% inhibition Cytophaga sp.
Hg2+ 2 mM, 98% inhibition Cytophaga sp.
iodoacetate 1 mM, 94% inhibition Cytophaga sp.
K+ 2 mM, 98% inhibition Cytophaga sp.
N-ethylmaleimide 1 mM, 100% inhibition Cytophaga sp.
Na+ 2 mM, 98% inhibition Cytophaga sp.
Ni2+ 2 mM, 98% inhibition Cytophaga sp.
p-chloromercuribenzoate 0.1 mM, 100% inhibition Cytophaga sp.
Semicarbazide hydrochloride 1 mM, 30% inhibition Cytophaga sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.797
-
 fumarate 0.1 mM, 100% inhibition Cytophaga sp.
1.09
-
 L-aspartate pH 7.0, 55°C Cytophaga sp.
25.2
-
 NH3 0.1 mM, 100% inhibition Cytophaga sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation at neutral pH Cytophaga sp.
Mg2+ activation at neutral pH Cytophaga sp.
Mn2+ activation at neutral pH Cytophaga sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 4 * 51000, SDS-PAGE Cytophaga sp.
192000
-
 gel filtration Cytophaga sp.

Organism

Organism UniProt Comment Textmining
Cytophaga sp.
-
 KUC-1
-
Cytophaga sp. KUC-1
-
 KUC-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cytophaga sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
301
-
 pH 8.5, 30°C Cytophaga sp.

Storage Stability

Storage Stability Organism
-20°C, pH 7.0, 2% 2-mercaptoethanol, several months, no loss of activity Cytophaga sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + hydroxylamine at 64% the rate of NH3 Cytophaga sp. L-aspartate
-
 ?
fumarate + hydroxylamine at 64% the rate of NH3 Cytophaga sp. KUC-1 L-aspartate
-
 ?
fumarate + NH3
-
 Cytophaga sp. L-aspartate
-
 ?
fumarate + NH3
-
 Cytophaga sp. KUC-1 L-aspartate
-
 ?
L-aspartate
-
 Cytophaga sp. fumarate + NH3
-
 r
L-aspartate
-
 Cytophaga sp. KUC-1 fumarate + NH3
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 51000, SDS-PAGE Cytophaga sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Cytophaga sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45
-
 Cytophaga sp.
50
-
 60 min, 80% residual activity Cytophaga sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 absence of Mg2+ Cytophaga sp.
8.5
-
 presence of Mg2+ Cytophaga sp.