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Literature summary for 4.3.1.1 extracted from
- Enhancement of the activity of l-aspartase from Escherichia coli W by directed evolution (2000), Biochem. Biophys. Res. Commun., 276, 346-349.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutant N217K/T233R/V367G through enzyme modification by direct evolution in order to enhance enzymic activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
L-aspartate | wild type | Escherichia coli |  |
| 2.6 | - |
L-aspartate | mutant N217K/T233R/V367G | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | - |
Escherichia coli | fumarate + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
wild type | Escherichia coli |
| 8.2 | - |
mutant N217K/T233R/V367G | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | mutant N217K/T233R/V367G | Escherichia coli |
| 7.8 | 9.3 | evolved enzyme | Escherichia coli |
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