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Literature summary for 4.3.1.1 extracted from
- Extractive purification of aspartase from Escherichia coli K12 (1994), Methods Enzymol., 228, 590-599.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme or whole cells are used for the industrial production of L-aspartate, which together with L-phenylalanine is the basis of the sweetener aspartame | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 strain JRG 1849 | - |
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| renaturation by dialysis against 50 mM Tris-HCl buffer ,pH 8.0, at 4C for 2 days | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| 219 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | r | Escherichia coli | fumarate + NH3 | - |
? |  |
| L-aspartate | r | Pseudomonas fluorescens | fumarate + NH3 | - |
? | |
| L-aspartate | natural substrate | Escherichia coli | fumarate + NH3 | - |
? | |
| L-aspartate | natural substrate | Pseudomonas fluorescens | fumarate + NH3 | - |
? | |
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