Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Tulipa gesneriana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Tulipa gesneriana | |
Cu2+ | - |
Tulipa gesneriana | |
Fe2+ | - |
Tulipa gesneriana | |
Hg2+ | - |
Tulipa gesneriana | |
phenylmethylsulfonyl fluoride | 2 mM inhibits the activity of isoforms 1, 2 and 3 by 89, 99.7 and 90.2%, respectively | Tulipa gesneriana | |
V3+ | - |
Tulipa gesneriana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
6-tuliposide A | isoform 2, pH and temperature not specified in the publication | Tulipa gesneriana | |
1.8 | - |
6-tuliposide A | isoform 1, pH and temperature not specified in the publication | Tulipa gesneriana | |
3 | - |
6-tuliposide A | isoform 3, pH and temperature not specified in the publication | Tulipa gesneriana | |
7.4 | - |
6-tuliposide B | isoform 3, pH and temperature not specified in the publication | Tulipa gesneriana | |
8.7 | - |
6-tuliposide B | isoform 2, pH and temperature not specified in the publication | Tulipa gesneriana | |
9.5 | - |
6-tuliposide B | isoform 1, pH and temperature not specified in the publication | Tulipa gesneriana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Tulipa gesneriana | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, isoform 1, 2, and 3, SDS-PAGE | Tulipa gesneriana |
92000 | - |
isoform 2, gel filtration | Tulipa gesneriana |
93000 | - |
isoform 3, gel filtration | Tulipa gesneriana |
99000 | - |
isoform 1, gel filtration | Tulipa gesneriana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-tuliposide B | Tulipa gesneriana | preferred substrate | tulipalin B + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tulipa gesneriana | - |
cultivar Murasakizuisho | - |
Purification (Comment) | Organism |
---|---|
butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration | Tulipa gesneriana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | the enzyme is specifically expressed in the pollen grains, but not in the anther cells | Tulipa gesneriana | - |
stamen | - |
Tulipa gesneriana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-tuliposide A | the activities of isoforms 1, 2 and 3 towards 6-tuliposide A are 0.41, 1.8, and 1.8% of those towards P6-tuliposide B, respectively | Tulipa gesneriana | tulipalin A + D-glucose | - |
? | |
6-tuliposide B | preferred substrate | Tulipa gesneriana | tulipalin B + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47000, isoform 1, 2, and 3, SDS-PAGE | Tulipa gesneriana |
Synonyms | Comment | Organism |
---|---|---|
PosB-converting enzyme | - |
Tulipa gesneriana |
TCEB | - |
Tulipa gesneriana |
TCEB1 | - |
Tulipa gesneriana |
tuliposide B-converting enzyme | - |
Tulipa gesneriana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Tulipa gesneriana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | the stable temperatures of the enzymes are up to 45°C for isoforms 1 and 2, and 50°C for isoform 3 | Tulipa gesneriana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Tulipa gesneriana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | isoforms 1, 2 and 3 are stable in the pH range of 4.5-10.0 | Tulipa gesneriana |