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Literature summary for 4.2.99.21 extracted from

  • Zhou, Y.; Memelink, J.; Linthorst, H.J.M.
    An E. coli biosensor for screening of cDNA libraries for isochorismate pyruvate lyase-encoding cDNAs (2018), Mol. Genet. Genomics, 293, 1181-1190 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.21

Cloned(Commentary)

Cloned (Comment) Organism
gene prxr1, Arabidopsis thaliana cDNA library screening using XL1-LUX, recombinant expression of His-tagged PRXR1 protein in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information development of an Escherichia coli salicylate (SA) biosensor to screen for IPL activity based on the SalR regulator/psalA promoter combination from Acinetobacter sp. ADP1, to control the expression of the reporter luxCDABE. The sensing components, including the SA-inducible promoter PsalA, the reporter operon luxCDABE and the salR gene encoding the LysR-type regulator of salA, are subcloned from Acinetobacter ADPWH_lux into a plasmid vector. The biosensor is responsive to micromolar concentrations of exogenous SA, and to endogenous SA produced after transformation with a plasmid permitting IPTG-inducible expression of bacterial IPL in this biosensor strain. After screening a cDNA library constructed from turnip crinkle virus (TCV)-infected Arabidopsis thaliana ecotype Di-17, an enzyme, PRXR1, is identified as a putative IPL that converts isochorismate into SA Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isochorismate Arabidopsis thaliana
-
 salicylate + pyruvate
-
 ?
isochorismate Arabidopsis thaliana ecotype Di-17
-
 salicylate + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana ecotype Di-17
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PRXR1 protein from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isochorismate
-
 Arabidopsis thaliana salicylate + pyruvate
-
 ?
isochorismate
-
 Arabidopsis thaliana ecotype Di-17 salicylate + pyruvate
-
 ?
additional information incubation of chorismate with the combination of the recombinant proteins EntC (isochorismate synthase, EC 5.4.4.2) and His-PRXR1 results in enhanced levels of salicylate, in a His-PRXR1-dependent manner Arabidopsis thaliana ?
-
 ?
additional information incubation of chorismate with the combination of the recombinant proteins EntC (isochorismate synthase, EC 5.4.4.2) and His-PRXR1 results in enhanced levels of salicylate, in a His-PRXR1-dependent manner Arabidopsis thaliana ecotype Di-17 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, recombinant His-tagged PRXR1, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
IPL
-
 Arabidopsis thaliana
isochorismate pyruvate lyase
-
 Arabidopsis thaliana
PRXR1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function salicylic acid (SA) is an essential hormone for development and induced defense against biotrophic pathogens in plants. The formation of SA mainly derives from chorismate via isochorismate synthase (ICS, EC 5.4.4.2) and isochorismate pyruvate lyase (IPL, EC 4.2.99.21)-mediated steps in Arabidopsis thaliana Arabidopsis thaliana