Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate | Pseudomonas aeruginosa | - |
salicylate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene pchB | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isochorismate = salicylate + pyruvate | reaction mechanism with catalytic residue Lys42, modeling, overview | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? | |
isochorismate | elimination of pyruvate | Pseudomonas aeruginosa | salicylate + pyruvate | - |
? | |
additional information | PchB can also perform a nonphysiological role as a chorismate mutase, EC 4.1.3.40, albeit with considerably lower catalytic efficiency | Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isochorismate-pyruvate lyase | - |
Pseudomonas aeruginosa |
PchB | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | PchB is a structural homologue of the AroQ chorismate mutases | Pseudomonas aeruginosa |
metabolism | the enzyme is involved in siderophore pyochelin via salicylate biosynthesis | Pseudomonas aeruginosa |
additional information | structure-function relationship, biocatalysis of pericyclic reactions, overview. For PchB, the pericyclic reaction is a concerted but asynchronous [1,5]-sigmatropic shift with a quantitative transfer of hydrogen from C2 to C9. Major structural difference between the apo form and the pyruvate-bound or the pyruvate-and salicylate-bound forms of PchB: the active site loop between helix 1 and helix 2 is disordered in the apo structure but fully ordered in the ligand-bound structures. The difference between the open and closed structures is due to a conserved active site lysine 42, which hydrogen bonds to a bound pyruvate molecule. Quantum mechanical/molecular mechanical molecular dynamics simulations, overview | Pseudomonas aeruginosa |