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Literature summary for 4.2.99.21 extracted from

  • Mauch, F.; Mauch-Mani, B.; Gaille, C.; Kull, B.; Haas, D.; Reimmann, C.
    Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase (2001), Plant J., 25, 67-77.
    View publication on PubMed

Application

Application Comment Organism
agriculture expression of a fusion of genes pchA and pchB from Pseudomonas aeruginosa, which encode isochorismate synthase and isochorismate pyruvate-lyase, in Arabidopsis thaliana, with targeting of the gene product either to the cytosol, c-SAS plants, or to the chloroplast, p-SAS plants. In p-SAS plants, the amount of free and conjugated SA is increased more than 20fold above wild type level. P-SAS plants show a strongly dwarfed phenotype and produce very few seeds. Targeting of SAS to the cytosol causes a slight increase in free salicylic acid and a significant threefold increase in conjugated salicylic acid. The modest increase in total salicylic content does not strongly induce the resistance marker PR-1, but results in enhanced disease resistance towards a virulent isolate of Peronospora parasitica. Increased resistance of c-SAS lines is paralleled with reduced seed production Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression of a fusion of genes pchA and pchB from Pseudomonas aeruginosa, which encode isochorismate synthase and isochorismate pyruvate-lyase, in Arabidopsis thaliana Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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