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Literature summary for 4.2.99.21 extracted from
- Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis (2007), Biochemistry, 46, 954-964.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| native protein and selenomethionine-derivative, to 2.5-3.2 A resolution | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
isochorismate | pH 7.0, 37°C | Mycobacterium tuberculosis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the salicylate and isochorismate synthase activities of MbtI are Mg2+-dependent, and in the absence of Mg2+, MbtI has a promiscuous chorismate mutase activity similar to that of the isochorismate pyruvate lyase, PchB, from Pseudomonas aeruginosa | Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isochorismate | - |
Mycobacterium tuberculosis | salicylate + pyruvate | - |
? | |
| additional information | isochorismate is a kinetically competent intermediate in the synthesis of salicylate from chorismate. At pH values below 7.5 isochorismate is the dominant product while above this pH value the enzyme converts chorismate to salicylate without the accumulation of isochorismate in solution. MbtI may exploit a sigmatropic pyruvate elimination mechanism | Mycobacterium tuberculosis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MbtI | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
isochorismate | pH 7.0, 37°C | Mycobacterium tuberculosis | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
at pH values below 7.5 isochorismate is the dominant product while above this pH value the enzyme converts chorismate to salicylate without the accumulation of isochorismate in solution | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.5 | - |
isochorismate | pH 7.0, 37°C | Mycobacterium tuberculosis | |
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