Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E96A | the mutant does not exhibit any nuclease activity against 88-nt RNA substrate | Homo sapiens |
H309N | the mutant does not exhibit any nuclease activity against 88-nt RNA substrate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | K+ is inhibitory to the native APE1 at 0.2-10 mM with approximately 5fold inhibition | Homo sapiens | |
K+ | K+ is inhibitory to the native APE1 at 0.2-10 mM with approximately 5fold inhibition | Rattus norvegicus | |
Mg2+ | 10-20 mM Mg2+ is inhibitory to the RNA-cleaving activity of APE1 | Homo sapiens | |
Mg2+ | 10-20 mM Mg2+ is inhibitory to the RNA-cleaving activity of APE1 | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000642 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | native enzyme | Rattus norvegicus | |
0.000424 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | recombinant enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | K+ significantly stimulates recombinant APE1 activity at 20 mM by approximately 1.3fold (compared with the absence of K+) | Homo sapiens | |
K+ | K+ significantly stimulates recombinant APE1 activity at 20 mM by approximately 1.3fold (compared with the absence of K+) | Rattus norvegicus | |
Mg2+ | ion concentrations ranging from 0.2 to 2 mM Mg2+ promotes catalysis, APE1 is enhanced approximately 2fold (compared with the absence of Mg2+) in the presence of 2 mM Mg2+ | Homo sapiens | |
Mg2+ | ion concentrations ranging from 0.2 to 2 mM Mg2+ promotes catalysis, APE1 is enhanced approximately 2fold (compared with the absence of Mg2+) in the presence of 2 mM Mg2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, SP-Sepharose column chromatography | Homo sapiens |
Ni-NTA column chromatography, SP-Sepharose column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate OligoI is based on the sequence immediately surrounding the stem V-loop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide | Homo sapiens | ? | - |
? | |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate OligoI is based on the sequence immediately surrounding the stem V-loop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide | Rattus norvegicus | ? | - |
? | |
5'-Cy3-CAAGGTAGTTATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate DNAOligoI is based on the sequence immediately surrounding the stem Vloop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide, DNAOligoI has an identical sequence to OligoI except that deoxythymidylate is substituted for 2' hydroxyl uridine | Homo sapiens | ? | - |
? | |
5'-Cy3-CAAGGTAGTTATCCTTG-1-Black Hole Quencher1-3' | the fluorogenic substrate DNAOligoI is based on the sequence immediately surrounding the stem Vloop region (OligoI) and incorporating a fluorescent tag, Cy3, at the 5' end and a fluorescence Black Hole Quencher at the 3' end of the oligonucleotide, DNAOligoI has an identical sequence to OligoI except that deoxythymidylate is substituted for 2' hydroxyl uridine | Rattus norvegicus | ? | - |
? | |
c-myc RNA | APE1 cleaves at the UA, CA, and UG sites of c-myc RNA in vitro | Homo sapiens | ? | - |
? | |
c-myc RNA | APE1 cleaves at the UA, CA, and UG sites of c-myc RNA in vitro | Rattus norvegicus | ? | - |
? | |
additional information | APE1 at low concentrations does not have any effect on 5'-Cy3-CrUAGGTAGTTATCCrUAG-Black Hole Quencher1-3' (OligoII), under similar conditions, the recombinant APE1 has no effect on fluorescently labeled or 32P-labeled DNAOligoI | Homo sapiens | ? | - |
? | |
additional information | APE1 at low concentrations does not have any effect on 5'-Cy3-CrUAGGTAGTTATCCrUAG-Black Hole Quencher1-3' (OligoII), under similar conditions, the recombinant APE1 has no effect on fluorescently labeled or 32P-labeled DNAOligoI | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1 | - |
Homo sapiens |
APE1 | - |
Rattus norvegicus |
apurinic-apyrimidinic endonuclease I | - |
Homo sapiens |
apurinic-apyrimidinic endonuclease I | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0272 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | recombinant enzyme | Homo sapiens | |
0.168 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | native enzyme | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | in addition to the apurinic/apyrimidinic DNA endonuclease activity, APE1 has 3'-5' DNA exonuclease, 3' phosphodiesterase, and RNase H activities | Rattus norvegicus |
physiological function | in addition to the apurinic/apyrimidinic DNA endonuclease activity, APE1 has 3'-5' DNA exonuclease, 3'-phosphodiesterase, and RNase H activities | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.15 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | recombinant enzyme | Homo sapiens | |
2620 | - |
5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3' | native enzyme | Rattus norvegicus |