Cloned (Comment) | Organism |
---|---|
repression of APE/Ref-1 by an antisense overexpression, transfection of mouse B-cells with phosphorothioate oligonucleotide, which is complementary to the APE/Ref-1 mRNA sequence and overlaps the translation initiation site (antisense oligonucleotide) | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
additional information | APE/Ref-1 is equally distributed between the nucleus and the cytoplasm, whereas at 24 hours, the CD40-induced-B cell activation relocalized APE/Ref-1 in the nucleus | Mus musculus | - |
- |
nucleus | - |
Mus musculus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Mus musculus | enzyme plays an important role in oxidative signalling, transcription factor regulation, and cell cycle control | fragments of DNA | - |
ir | |
additional information | Mus musculus | APE/Ref-1 act as tuning molecule in activated B-cells | ? | - |
? | |
additional information | Mus musculus | APE/Ref-1 affect the cell cycle by inducing nucleus-cytoplasm translocation of the cyclin-dependent kinase inhibitor p21 | ? | - |
? | |
additional information | Mus musculus | enzyme plays a role in controlling CD40-mediated B-cell proliferation, increase in proliferation and decrease in apoptosis of primary mouse B-cells activated by CD40 cross-linking and transfected with functional APE/Ref-1 antisense oligonucleotide. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte cell line | primary cell line, murine B-cells are isolated from spleen by a negative depletion | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the redox activity of APE/Ref-1 is regulated by chemical reduction and oxidation in vitro | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | enzyme plays an important role in oxidative signalling, transcription factor regulation, and cell cycle control | Mus musculus | fragments of DNA | - |
ir | |
additional information | APE/Ref-1 act as tuning molecule in activated B-cells | Mus musculus | ? | - |
? | |
additional information | APE/Ref-1 affect the cell cycle by inducing nucleus-cytoplasm translocation of the cyclin-dependent kinase inhibitor p21 | Mus musculus | ? | - |
? | |
additional information | enzyme plays a role in controlling CD40-mediated B-cell proliferation, increase in proliferation and decrease in apoptosis of primary mouse B-cells activated by CD40 cross-linking and transfected with functional APE/Ref-1 antisense oligonucleotide. | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme has a DNA repair domain at the C-terminus and a redox regulation domain at the N-terminus | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
APE/Ref-1 | - |
Mus musculus |
apurinic/apyrimidinic endonuclease-1/Redox factor-1 | multifunctional DNA base excision repair and redox regulation enzyme | Mus musculus |