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Literature summary for 4.2.99.18 extracted from

  • Zhao, J.; Gao, F.; Zhang, Y.; Wei, K.; Liu, Y.; Deng, X.
    Bcl2 inhibits abasic site repair by down-regulating APE1 endonuclease activity (2008), J. Biol. Chem., 283, 9925-9932.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Inhibitors

Inhibitors Comment Organism Structure
proteinBcl2 overexpression of Bcl2, a major cellular oncogenic protein, in cells reduces formation of the APE1-XRCC1 complex, Bcl2 not only prolongs cell survival but also suppresses the repair of abasic (AP) sites of DNA lesions. Bcl2 directly interacts with APE1 via its BH domains, and deletion of any of the BH domains from Bcl2 results in loss of the ability of Bcl2 to suppress APE1 endonuclease activity and AP site repair Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ included in assay medium Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens APE1 is also named as redox effector factor-1 because of its redox abilities on different redox-regulated transcription factors ?
-
 ?
additional information Homo sapiens human apurinic/apyrimidinic endonuclease 1 is a major constituent of the base excision repair (BER) pathway of AP sites of DNA lesions. APE1 specifically binds to abasic sites and cuts the 5'-phosphodiester bond with its endonuclease activity to produce a DNA primer with 3'-hydroxyl end, which is a required step in the BER repair pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation purified recombinant APE1 protein Homo sapiens
-
lung cancer cell line various cell lines, Bcl2, a major antiapoptotic and/or oncogenic protein, is found to co-express with APE1 in H69 and H460 but not in other tested lung cancer cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 decreased levels of AP endonuclease activity (smaller amount of cleaved 14-mer products and greater amount of uncleaved 26-mer oligonucleotides) are observed in both H460 and H69 cells that express high levels of endogenous Bcl2 as compared with the other cell lines that express undetectable levels of Bcl2 Homo sapiens
additional information
-
 expression of endogenous Bcl2 is associated with decreased APE1 endonuclease activity in various human lung cancer cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information APE1 is also named as redox effector factor-1 because of its redox abilities on different redox-regulated transcription factors Homo sapiens ?
-
 ?
additional information human apurinic/apyrimidinic endonuclease 1 is a major constituent of the base excision repair (BER) pathway of AP sites of DNA lesions. APE1 specifically binds to abasic sites and cuts the 5'-phosphodiester bond with its endonuclease activity to produce a DNA primer with 3'-hydroxyl end, which is a required step in the BER repair pathway Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
APE1
-
 Homo sapiens
apurinic/apyrimidinic endonuclease 1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens