Literature summary for 4.2.99.18 extracted from

Futagami, S.; Hiratsuka, T.; Shindo, T.; Horie, A.; Hamamoto, T.; Suzuki, K.; Kusunoki, M.; Miyake, K.; Gudis, K.; Crowe, S.E.; Tsukui, T.; Sakamoto, C.
Expression of apurinic/apyrimidinic endonuclease-1 (APE-1) in H. pylori-associated gastritis, gastric adenoma, and gastric cancer (2008), Helicobacter, 13, 209-218.

Search for more literature for 4.2.99.18

Activating Compound

Activating Compound	Comment	Organism	Structure
Helicobacter pylori (CagA+) water-extract protein (HPWEP)	HPWEP-stimulation significantly increases APE-1 mRNA expression levels in human peripheral macrophages. HPWEP-stimulation increases APE-1 expression levels in AGS cells. HPWEP stimulation increases APE-1 protein expression in gastric epithelial MKN-28 cells in a dose-dependent manner (1:30 and 1:10 dilution). When normalized to beta-actin, HPWEP stimulation at a 1:10 dilution significantly increases APE-1 protein expression compared to control MKN-28 cells. APE-1 protein expression is further increased in HPWEP (1:10 dilution) stimulated MKN-28 cells after treatment with hypo/reoxygenation.	Homo sapiens

Application

Application	Comment	Organism
medicine	levels of APE-1 expression in gastric and other cancer tissues may be used to determine chemosensitivity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
APE-1 proteins partially purified purified are visualized by Western blot using anti-human APE-1 antibody	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
AGS cell	gastric adenocarcinoma cell-line	Homo sapiens	-
epithelial cell	APE-1 expression is mainly localized in epithelial cells within gastric adenoma	Homo sapiens	-
macrophage	human peripheral macrophages	Homo sapiens	-
mesenchyme	APE-1 expression in gastric cancer tissues	Homo sapiens	-
MKN-28 cell	Helicobacter pylori (CagA+) water-extract protein (HPWEP)-stimulated	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	APE-1 expression in tissues from Helicobacter pylori-infected subjects is significantly increased compared to that in tissues from subjects after successful eradication therapy	Homo sapiens

Synonyms

Synonyms	Comment	Organism
APE-1	-	Homo sapiens
apurinic/apyrimidinic endonuclease-1	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)