Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cisplatin | cisplatin-induced (chemotherapeutic agent) increase in Api1 expression after exposition with 25 micromol/l or higher concentrations for 24 hours, Api1 is neuroprotective against cisplatin-induced neurotoxity | Rattus norvegicus | |
additional information | after infection with adenoviral constructs (mutant C65A) and treatment with 50 micromol/l cisplatin, Ape1 is overexpressed in sensory neuronal rat cell cultures | Rattus norvegicus |
Application | Comment | Organism |
---|---|---|
drug development | cancer therapy | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
neuronal cell cultures are transfected with Api1 small interfering RNA, rat neuronal cell cultures are infected with adenovirus | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C65A | redox deficient-DNA repair competent (adenovirus infected) | Rattus norvegicus |
N226A/R177A | DNA repair-deficient-redox competent Ape1 does not protect rat neurons from cisplatin toxicity (adenovirus infected) | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | exposing neuronal cultures to Api1 small interfering RNA significantly reduces the expression of Api1 | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Rattus norvegicus | multifunctional enzyme involved in DNA base excision repair of oxidative DNA damage and in redox regulation of a number of transcription factors | fragments of DNA | - |
? | |
additional information | Rattus norvegicus | increase in phosphorylation of p53 after a decrease in Ape1 levels in sensory neuronal cultures | ? | - |
? | |
additional information | Rattus norvegicus | overexpressing wild-type Ape1 attentuates all the toxic effects of cisplatin in cells containing normal endogenous levels of Ape1 and in cells with reduced Ape1 levels after Ape1siRNA treatment | ? | - |
? | |
additional information | Rattus norvegicus | reducing expression of Ape1 in neuronal cultures using small interfering RNA enhances cisplatin-induced cell killing, apoptosis, ROS generation and cisplatin-induced reduction in iCGRP release | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ganglioneuroma cell, dorsal root ganglions are collected from the spinal column, incubated with collagenase and mechanically dissociated | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
overexpression of Ape1 reduces cisplatin-induced apoptosis in sensory neuronal cell cultures | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | multifunctional enzyme involved in DNA base excision repair of oxidative DNA damage and in redox regulation of a number of transcription factors | Rattus norvegicus | fragments of DNA | - |
? | |
additional information | increase in phosphorylation of p53 after a decrease in Ape1 levels in sensory neuronal cultures | Rattus norvegicus | ? | - |
? | |
additional information | overexpressing wild-type Ape1 attentuates all the toxic effects of cisplatin in cells containing normal endogenous levels of Ape1 and in cells with reduced Ape1 levels after Ape1siRNA treatment | Rattus norvegicus | ? | - |
? | |
additional information | reducing expression of Ape1 in neuronal cultures using small interfering RNA enhances cisplatin-induced cell killing, apoptosis, ROS generation and cisplatin-induced reduction in iCGRP release | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE/Ref-1 | - |
Rattus norvegicus |
APE1 | - |
Rattus norvegicus |
apurinic/apyrimidinic endonuclease/redox effector factor-1 | - |
Rattus norvegicus |