Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lipopolysaccharide | 100 ng/ml lipopolysaccharide stimulates the upregulation and nuclear translocation of APE1 in activated macrophages. APE1 critically mediates both the translocation of NF-B to the nucleus and the expression of inducible nitric oxide synthase by murine macrophage RAW264.7 cells after stimulation with LPS | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | cancer therapy, inflammatory diseases | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
wild-type APE1 encoding full-length of APE1 and APE1 C65A/C93A encoding mutant form of APE1 in pCMVTag2B mammalian expression vector are generated by standard cloning method. TAT-APE1 is generated by insertion of full-length of APE1 into pTAT-2.1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C65A/C93A | mutant form of TAT-APE1 is generated by insertion of full-length of APE1 C65A/C93A into pTAT-2.1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | APE1 silencing via siRNA transfection inhibits both the nuclear and cytoplasmic expression of APE1 | Homo sapiens | |
polyinosinic-polycytidylic acid | transfection of APE1 suppresses the extracellular release of high-mobility group box 1 in response to polyinosinic-polycytidylic acid stimulation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | weak and diffuse cytosolic distribution under basal conditions | Homo sapiens | 5737 | - |
additional information | high-mobility group box 1 stimulation induces the nuclear translocation of APE1 protein, and siRNA transfection and adenoviral transduction results in differential expression and localization patterns in activated macrophages | Homo sapiens | - |
- |
nucleus | When THP-1 cells are incubated with high-mobility group box 1 for 12 h, cytoplasmic APE1 proteins are substantially translocated into nucleus. APE1 proteins are significantly translocated from cytoplasmic to nuclear fractions in THP-1 cells at 24 h after high-mobility group box 1 stimulation. Overexpression of APE1 in human primary monocytes with adenoviral transduction leads to abundant protein expression in the cytoplasmic and nuclear fractions. | Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | forced cytoplasmic overexpression of APE1 profoundly attenuates the upregulation of high-mobility group box 1-mediated reactive oxygen species generation, cytokine secretion, and cyclooxygenase-2 expression by primary monocytes and macrophage-like THP-1 cell lines | ? | - |
? | |
additional information | Homo sapiens | high-mobility group box 1-induced activation of p38 and c-Jun N-terminal kinase is strongly abrogated by the overexpression of APE1 | ? | - |
? | |
additional information | Homo sapiens | multifunctional protein involved in reduction-oxidation regulation. It functions as a redox factor that maintains transcription factors in an active reduced state | ? | - |
? | |
additional information | Homo sapiens | small interfering RNA knockdown of endogenous APE1 impairs high-mobility group box 1-mediated cytokine expression and MAPK activation in THP-1 cells. High-mobility group box 1-stimulation induces the translocation of APE1 to the nucleus of the cell | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
monocyte | prepared from peripheral blood mononuclear cells | Homo sapiens | - |
THP-1 cell | human acute monocytic leukemia cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | forced cytoplasmic overexpression of APE1 profoundly attenuates the upregulation of high-mobility group box 1-mediated reactive oxygen species generation, cytokine secretion, and cyclooxygenase-2 expression by primary monocytes and macrophage-like THP-1 cell lines | Homo sapiens | ? | - |
? | |
additional information | high-mobility group box 1-induced activation of p38 and c-Jun N-terminal kinase is strongly abrogated by the overexpression of APE1 | Homo sapiens | ? | - |
? | |
additional information | multifunctional protein involved in reduction-oxidation regulation. It functions as a redox factor that maintains transcription factors in an active reduced state | Homo sapiens | ? | - |
? | |
additional information | small interfering RNA knockdown of endogenous APE1 impairs high-mobility group box 1-mediated cytokine expression and MAPK activation in THP-1 cells. High-mobility group box 1-stimulation induces the translocation of APE1 to the nucleus of the cell | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1 | - |
Homo sapiens |
apurinic/apyrimidinic endonuclease 1/redox factor-1 | - |
Homo sapiens |