Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli | Pyrococcus abyssi |
Crystallization (Comment) | Organism |
---|---|
structures are deposited into the Brookhaven Protein Data Bank under the accession numbers 2IVN, 2IVO and 2IVP, structure is solved to 3.0 A and further refined to 1.6 A | Pyrococcus abyssi |
Protein Variants | Comment | Organism |
---|---|---|
Y127F | decreased endonuclease activity, Tyr 127 is essential to the binding and stabilization of the Fe3+ ion, mutation do not affect DNA binding | Pyrococcus abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus abyssi | |
EDTA | - |
Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | - |
Pyrococcus abyssi | |
Mg2+ | - |
Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36200 | - |
recombinant histidine-tagged Pa-Kae1 | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Subunits | Comment | Organism |
---|---|---|
monomer | the purified protein is present as a monomer in solution | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
AP-endonuclease | - |
Pyrococcus abyssi |
AP-lyase | - |
Pyrococcus abyssi |
apurinic endonuclease | - |
Pyrococcus abyssi |
Kae1 | kinase-associated endopeptidase 1 exibits a class I apurinic endonuclease activity, no activity in vitro | Pyrococcus abyssi |
PaKae1 | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
DNA-binding assay | Pyrococcus abyssi |
72 | - |
AP-endonuclease assay | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
AP-endonuclease assay | Pyrococcus abyssi |
7.5 | - |
DNA-binding assay | Pyrococcus abyssi |