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Literature summary for 4.2.99.18 extracted from

  • Carpenter, E.P.; Corbett, A.; Thomson, H.; Adacha, J.; Jensen, K.; Bergeron, J.; Kasampalidis, I.; Exley, R.; Winterbotham, M.; Tang, C.; Baldwin, G.S.; Freemont, P.
    AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis (2007), EMBO J., 26, 1363-1372.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment) Organism
into the pProEX HTb vector for transformation of Escherichia coli XL-1 Blue and ER2566 cells Neisseria meningitidis

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of NMB2082/NApe is solved at a 1.5 A resolution Neisseria meningitidis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the standard reaction buffer used for the nuclease assay is supplemented with 15 mM MgCl2 Neisseria meningitidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 determined by SDS-PAGE and Western blotting Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a metal-affinity HisTrap chelating HP column, preequilibrated with CoCl2, and size-exclusion chromatography, using a HiLoad 16/60 Superdex 200 prep grade column Neisseria meningitidis

Synonyms

Synonyms Comment Organism
AP endonuclease
-
 Neisseria meningitidis
apurinic/apyrimidinic endonuclease
-
 Neisseria meningitidis
NapE Neisserial AP endonuclease Neisseria meningitidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 nuclease assay Neisseria meningitidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 nuclease assay Neisseria meningitidis