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Literature summary for 4.2.99.18 extracted from

  • Nakabeppu, Y.; Sekiguchi, M.
    Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA (1981), Proc. Natl. Acad. Sci. USA, 78, 2742-2746.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 bacteriophage T4 infected, SDS-PAGE, T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease EC 4.2.99.18 activity in a single polypeptide chain Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Escherichia coli
-
 fragments of DNA
-
 ?
DNA Tequatrovirus T4
-
 fragments of DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 bacteriophage T4 infected, T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease (EC 4.2.99.18) activity in a single polypeptide chain
-
Tequatrovirus T4
-
 Escherichia coli infected with bacteriophage T4, T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease (E.C. 3.1.25.2) activity in a single polypeptide chain
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4
infected with bacteriophage T4 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate T4 endonuclease V (EC 3.1.25.1) possesses both pyrimidine dimer DNA glycosylase activity and apurinic/apyrimidinic DNA endonuclease (EC 4.2.99.18) activity in a single polypeptide chain Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
 Escherichia coli fragments of DNA
-
 ?
DNA
-
 Tequatrovirus T4 fragments of DNA
-
 ?
DNA endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphates, e.g. UV-irradiated poly(dA)*poly(dT) Escherichia coli fragments of DNA
-
 ?
DNA endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphates, e.g. UV-irradiated poly(dA)*poly(dT) Tequatrovirus T4 fragments of DNA
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 5 min, 70% loss of activity Escherichia coli
42
-
 5 min, 70% loss of activity Tequatrovirus T4