Cloned (Comment) | Organism |
---|---|
gene CsTPS13PK, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis | Cannabis sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cannabis sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Cannabis sativa | - |
(Z)-beta-ocimene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cannabis sativa | A0A1V0QSG1 | var. Finola | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | transcriptome analysis of trichomes of the cannabis hemp variety Finola reveals sequences of all stages of terpene biosynthesis. Major terpene compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene. Transcripts associated with terpene biosynthesis are highly expressed in trichomes compared to non-resin producing tissues. Relative composition of terpene profiles in Cannabis sativa var. Finola pistillate flowers, overview | Cannabis sativa | - |
trichome | glandular | Cannabis sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Cannabis sativa | (Z)-beta-ocimene + diphosphate | - |
? | |
geranyl diphosphate | preferred substrate | Cannabis sativa | (Z)-beta-ocimene + diphosphate | - |
? | |
additional information | the enzyme also shows low activity with farnesyl diphosphate. Reaction products are identified by GC-MS analysis | Cannabis sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(Z)-beta-ocimene synthase | - |
Cannabis sativa |
CsTPS13PK | - |
Cannabis sativa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the terpene synthase (TPS) superfamily, CsTPSFN TPS-b subfamily. Gene CsTPS13PK, encoding (Z)-beta-ocimene synthase, shares 95.5% amino acid sequence identity with gene CsTPS6FN, encoding (E)-beta-ocimene synthase | Cannabis sativa |
metabolism | transcriptome analysis of trichomes of the cannabis hemp variety Finola reveal sequences of all stages of terpene biosynthesis. Nine cannabis terpene synthases (CsTPS) are identified in subfamilies TPS-a and TPS-b. Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of Finola resin, including major compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene | Cannabis sativa |