Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Nostoc sp. |
expressed in Escherichia coli BL21(DE3) cells | Nostoc punctiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
(E,E)-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C | Nostoc punctiforme | |
0.0034 | - |
(2E,6E)-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C | Nostoc sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
- |
- |
Nostoc sp. | - |
- |
- |
Nostoc sp. PCC 712 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity column chromatography | Nostoc sp. |
Talon metal affinity column chromatography | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Nostoc sp. | 8a-epi-alpha-selinene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Nostoc sp. PCC 712 | 8a-epi-alpha-selinene + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate | - |
Nostoc punctiforme | 8a-epi-alpha-selinene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NP1 | - |
Nostoc punctiforme |
NS1 | - |
Nostoc sp. |
terpene synthase | - |
Nostoc sp. |
terpene synthase | - |
Nostoc punctiforme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
(2E,6E)-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C | Nostoc sp. | |
0.063 | - |
(E,E)-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C | Nostoc punctiforme |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13000 | - |
(2E,6E)-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C | Nostoc sp. | |
24000 | - |
(E,E)-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C | Nostoc punctiforme |