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Literature summary for 4.2.3.9 extracted from

  • Chen, M.; Chou, W.K.; Al-Lami, N.; Faraldos, J.A.; Allemann, R.K.; Cane, D.E.; Christianson, D.W.
    Probing the role of active site water in the sesquiterpene cyclization reaction catalyzed by aristolochene synthase (2016), Biochemistry, 55, 2864-2874 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.9

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzymes in complex with (2E,6E)-farnesyl diphosphate or 4, sitting drop vapor diffusion method, using 20% PEG 3350 as precipitant Aspergillus terreus

Protein Variants

Protein Variants Comment Organism
N299A the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions of 50% germacrene A and 50% aristolochene Aspergillus terreus
N299A/S303A the decreasing have modest effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A as main product and (E)-nerolidol and aristolochene as by-products Aspergillus terreus
N299L the mutation has decreasing effects on sesquiterpene product distributions with germacrene A and aristolochene as main product and (E)-nerolidol as by-product Aspergillus terreus
N299V inactive Aspergillus terreus
Q151E the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with aristolochene and germacrene A as main products, and (E)-nerolidol and (E,E)-farnesol as by-products Aspergillus terreus
Q151H the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A and (E,E)-farnesol as main products and (E)-nerolidol and aristolochene as by-products Aspergillus terreus
S303A the mutation has increasing effects on catalysis and significant effects on sesquiterpene product distributions with germacrene A and (E)-nerolidol as by-products Aspergillus terreus
S303D the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A and (E,E)-farnesol as main products and (E)-nerolidol and aristolochene as by-products Aspergillus terreus
S303H the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A as main product and (E,E)-farnesol, (E)-nerolidol and aristolochene as by-products Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000042
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151H, at pH 8.0 and 30°C Aspergillus terreus
0.000061
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303H, at pH 8.0 and 30°C Aspergillus terreus
0.000099
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 8.0 and 30°C Aspergillus terreus
0.0001
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151E, at pH 8.0 and 30°C Aspergillus terreus
0.00012
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303A, at pH 8.0 and 30°C Aspergillus terreus
0.00036
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303D, at pH 8.0 and 30°C Aspergillus terreus
0.00041
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A, at pH 8.0 and 30°C Aspergillus terreus
0.00041
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A/S303A, at pH 8.0 and 30°C Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Aspergillus terreus
-
 aristolochene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus Q9UR08
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 26/60 gel filtration Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Aspergillus terreus aristolochene + diphosphate
-
 ?
additional information no activity with farnesyl S-thiolodiphosphate Aspergillus terreus ?
-
 ?

Synonyms

Synonyms Comment Organism
ATAS
-
 Aspergillus terreus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00041
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303D, at pH 8.0 and 30°C Aspergillus terreus
0.00063
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151H, at pH 8.0 and 30°C Aspergillus terreus
0.0013
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303H, at pH 8.0 and 30°C Aspergillus terreus
0.0015
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151E, at pH 8.0 and 30°C Aspergillus terreus
0.0058
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A/S303A, at pH 8.0 and 30°C Aspergillus terreus
0.012
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A, at pH 8.0 and 30°C Aspergillus terreus
0.027
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 8.0 and 30°C Aspergillus terreus
0.045
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303A, at pH 8.0 and 30°C Aspergillus terreus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303D, at pH 8.0 and 30°C Aspergillus terreus
14
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A/S303A, at pH 8.0 and 30°C Aspergillus terreus
15
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151E, at pH 8.0 and 30°C Aspergillus terreus
15
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151H, at pH 8.0 and 30°C Aspergillus terreus
21
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303H, at pH 8.0 and 30°C Aspergillus terreus
29
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A, at pH 8.0 and 30°C Aspergillus terreus
270
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 8.0 and 30°C Aspergillus terreus
370
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303A, at pH 8.0 and 30°C Aspergillus terreus