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Literature summary for 4.2.3.9 extracted from
- The role of aristolochene synthase in diphosphate activation (2012), Chem. Commun. (Camb. ), 48, 3230-3232.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D203L | approximately 150fold decrease in kcat/KM | Penicillium roqueforti |
| K251Q | 20fold reduction in catalytic efficiency | Penicillium roqueforti |
| K251R | 6fold reduction in catalytic efficiency | Penicillium roqueforti |
| R200E | inactive | Penicillium roqueforti |
| R200K | approximately 300fold decrease in kcat/KM, mutant produces significantly increased amounts of germacrene A | Penicillium roqueforti |
| R200Q | inactive | Penicillium roqueforti |
| R340K | approximately 300fold decrease in kcat/KM, mutant produces significantly increased amounts of germacrene A | Penicillium roqueforti |
| R340M | inactive | Penicillium roqueforti |
| Y341F | 10fold reduction in catalytic efficiency | Penicillium roqueforti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00014 | - |
(2E,6E)-farnesyl diphosphate | mutant K251R, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti |  |
| 0.00018 | - |
(2E,6E)-farnesyl diphosphate | mutant Y341F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00024 | - |
(2E,6E)-farnesyl diphosphate | mutant K251Q, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00053 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00074 | - |
(2E,6E)-farnesyl diphosphate | mutant D203L, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00104 | - |
(2E,6E)-farnesyl diphosphate | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00159 | - |
(2E,6E)-farnesyl diphosphate | mutant R340K, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium roqueforti | Q03471 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Penicillium roqueforti | (+)-5-epi-aristolochene + diphosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
(2E,6E)-farnesyl diphosphate | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00075 | - |
(2E,6E)-farnesyl diphosphate | mutant D203L, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0008 | - |
(2E,6E)-farnesyl diphosphate | mutant R340K, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.002 | - |
(2E,6E)-farnesyl diphosphate | mutant K251Q, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0025 | - |
(2E,6E)-farnesyl diphosphate | mutant Y341F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0037 | - |
(2E,6E)-farnesyl diphosphate | mutant K251R, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.084 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
(2E,6E)-farnesyl diphosphate | mutant R340K, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.62 | - |
(2E,6E)-farnesyl diphosphate | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 1.01 | - |
(2E,6E)-farnesyl diphosphate | mutant D203L, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 8.5 | - |
(2E,6E)-farnesyl diphosphate | mutant K251Q, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 14 | - |
(2E,6E)-farnesyl diphosphate | mutant Y341F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 26.4 | - |
(2E,6E)-farnesyl diphosphate | mutant K251R, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 158.5 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
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