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Literature summary for 4.2.3.9 extracted from
- Aristolochene synthase: purification, molecular cloning, high-level expression in Escherichia coli, and characterization of the Aspergillus terreus cyclase (2000), Arch. Biochem. Biophys., 376, 354-364.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| high-level expression in Escherichia coli | Aspergillus terreus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Aspergillus terreus |  |
| Mn2+ | above 1.0 mM | Aspergillus terreus | |
| Mn2+ | - |
Penicillium roqueforti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000135 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, native enzyme | Aspergillus terreus | |
| 0.0000148 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus terreus | |
| 0.00052 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, recombinant enzyme | Penicillium roqueforti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can substitute for Mg2+ over the concentration range of 0.16 -5.0 mM | Aspergillus terreus | |
| Mg2+ | - |
Penicillium roqueforti | |
| Mg2+ | divalent cation required, 5 mM Mg2+ preferred | Aspergillus terreus | |
| Mn2+ | - |
Penicillium roqueforti | |
| Mn2+ | Mn2+ can replace Mg2+ at 0.1-0.2 mM | Aspergillus terreus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36480 | - |
x * 36480, calculation from nucleotide sequence | Aspergillus terreus |
| 39000 | - |
x * 39000, SDS-PAGE | Aspergillus terreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | - |
- |
- |
| Penicillium roqueforti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant enzyme | Aspergillus terreus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0236 | - |
native enzyme | Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans,trans-farnesyl diphosphate | - |
Aspergillus terreus | aristolochene + diphosphate | - |
? | |
| trans,trans-farnesyl diphosphate | - |
Penicillium roqueforti | aristolochene + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Penicillium roqueforti |
| ? | x * 39000, SDS-PAGE | Aspergillus terreus |
| ? | x * 36480, calculation from nucleotide sequence | Aspergillus terreus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, native enzyme | Aspergillus terreus | |
| 0.015 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus terreus | |
| 0.043 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, recombinant enzyme | Penicillium roqueforti | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme, HEPES buffer | Aspergillus terreus |
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