Cloned (Comment) | Organism |
---|---|
gene CsSesquiTPS6b, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) | Citrus sinensis |
gene CsSesquiTPS6b, DNA and amino acid sequence determination and analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) | Citrus sinensis |
gene CsSesquiTPS6c, DNA and amino acid sequence determination and analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) | Citrus sinensis |
Protein Variants | Comment | Organism |
---|---|---|
A256E | site-directed mutagenesis of an alpha-helix residue disturbs the facing of the N-terminal loop | Citrus sinensis |
D319G | site-directed mutagenesis, the mutation prevents the interaction of this residue with one of the Mg2+ atoms | Citrus sinensis |
E41D | site-directed mutagenesis of an alpha-helix residue facing the N-terminal loop, the mutation can compensate the structural difference between enzymes CsSesquiTPS7 and CsSesquiTPS6b, both producing beta-caryophyllene and alpha-humulene | Citrus sinensis |
I260R | site-directed mutagenesis of an alpha-helix residue disturbs the facing of the N-terminal loop | Citrus sinensis |
T44P | site-directed mutagenesis of an alpha-helix residue facing the N-terminal loop, the mutation can compensate the structural difference between enzymes CsSesquiTPS7 and CsSesquiTPS6b, both producing beta-caryophyllene and alpha-humulene | Citrus sinensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, interaction between Mg2+ atoms and binding residues, structure overview | Citrus sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Citrus sinensis | - |
(+)-beta-caryophyllene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis | A0A067G476 | i.e. Citrus aurantium var. sinensis, pineapple sweet orange | - |
Citrus sinensis | A0A290U6M8 | i.e. Citrus aurantium var. sinensis, pineapple sweet orange | - |
Citrus sinensis | A0A290U6M9 | i.e. Citrus aurantium var. sinensis, pineapple sweet orange | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Citrus sinensis | - |
leaf | - |
Citrus sinensis | - |
additional information | in orange tissues, the sesquiterpene profile is qualitatively rich. Overall, up to 25 sesquiterpenes are identified in total, overview. Small amounts of beta-caryophyllene are detected in all samples, while alpha-copaene, beta-elemene, alpha-humulene, and alpha-selinene are emitted by all tissues except young leaves. Beside this, each tissue shows a characteristic sesquiterpene emission profile. Major sesquiterpenes emitted by adult leaves are beta-elemene and beta-caryophyllene, while flowers emit nerolidol, absent from most tissues analyzed. In fruit tissues, valencene emission contributes greatly to total emitted volatiles, in much more extent in peel than in pulp | Citrus sinensis | - |
additional information | in orange tissues, the sesquiterpene profile is qualitatively rich. Overall, up to 25 sesquiterpenes are identified in total, overview. Small amounts of beta-caryophyllene are detected in all samples, while alpha-copaene, beta-elemene, alpha-humulene, and alpha-selinene are emitted by all tissues except young leaves. Beside this, each tissue shows a characteristic sesquiterpene emission profile. Major sesquiterpenes emitted by adult leaves are beta-elemene and beta-caryophyllene, while flowers emitt nerolidol, absent from most tissues analyzed. In fruit tissues, valencene emission contributes greatly to total emitted volatiles, in much more extent in peel than in pulp | Citrus sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Citrus sinensis | (+)-beta-caryophyllene + diphosphate | - |
? | |
additional information | GC-MS analysis is used for compound identification | Citrus sinensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-caryophyllene synthase | - |
Citrus sinensis |
CsSesquiTPS6a | - |
Citrus sinensis |
CsSesquiTPS6b | - |
Citrus sinensis |
CsSesquiTPS6c | - |
Citrus sinensis |
SQS6a | - |
Citrus sinensis |
SQS6b | - |
Citrus sinensis |
SQS7 | - |
Citrus sinensis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the terpene synthase (TPS) family | Citrus sinensis |
additional information | three-dimensional structure homology modelling using the crystal structure of the delta-cadinene synthase from Gossypium. The functional C-terminal domain consists of 14 alpha-helices forming a central cavity and the amino terminal domain formed by two 10 antiparallel alpha-helices. The aspartate-rich motifs DDXXD (located on helix-alpha-13) and NSE/DTE motif (located helix alpha-20) are both involved in binding a trio of divalent magnesium ions, co-orientated to the catalytic pocket | Citrus sinensis |
additional information | three-dmensional structure homology modelling using the crystal structure of the delta-cadinene synthase from Gossypium. The functional C-terminal domain consists of 14 alpha-helices forming a central cavity and the amino terminal domain formed by two 10 antiparallel alpha-helices. The aspartate-rich motifs DDXXD (located on helix-alpha-13) and NSE/DTE motif (located helix alpha-20) are both involved in binding a trio of divalent magnesium ions, co-orientated to the catalytic pocket | Citrus sinensis |