Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | above 0.2 mM | Ricinus communis | |
additional information | 10 mM iodoacetamide is not inhibitory, SKF525A, N,N-dimethylaminoethyl-2,2-diphenylpentanoate and the growth retardant Amo-1618, 2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate are ineffective inhibitors | Ricinus communis | |
N-ethylmaleimide | activity is inhibited by 50% | Ricinus communis | |
Tributyl-2,4-dichlorobenzylphosphonium chloride | phosphonD, growth retardant, inhibits the activity by 55% | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
geranylgeranyl diphosphate | pH 9.0, 30°C | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Ricinus communis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highly dependent on, maximal stimulation of the activity above 5 mM, Mn2+ is much less effective | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
native enzyme, gel filtration | Ricinus communis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Ricinus communis | regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite | casbene + diphosphate | diterpene phytoalexin, product of a single enzyme-catalyzed transformation | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
Hale variety, N5 variety | - |
Ricinus communis | - |
castor bean, unknown variety | - |
Purification (Comment) | Organism |
---|---|
partial | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | infected with the fungus Rhizpus stolonifer, no detectable levels of casbene synthetase mRNA in healthy seedlings | Ricinus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0437 | - |
- |
Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Ricinus communis | casbene + diphosphate | - |
? | |
geranylgeranyl diphosphate | regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite | Ricinus communis | casbene + diphosphate | diterpene phytoalexin, product of a single enzyme-catalyzed transformation | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | half-maximal activity at pH 6.0 and 9.8 | Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.8 | half-maximal activity at pH 6.0 and 9.8 | Ricinus communis |