Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.8 extracted from

  • Dueber, M.T.; Adolf, W.; West, C.A.
    Biosynthesis of diterpene phytoalexin casbene: partial purification and characterization of casbene synthetase from Rhizinus communis (1978), Plant Physiol., 62, 598-603.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ above 0.2 mM Ricinus communis
additional information 10 mM iodoacetamide is not inhibitory, SKF525A, N,N-dimethylaminoethyl-2,2-diphenylpentanoate and the growth retardant Amo-1618, 2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate are ineffective inhibitors Ricinus communis
N-ethylmaleimide activity is inhibited by 50% Ricinus communis
Tributyl-2,4-dichlorobenzylphosphonium chloride phosphonD, growth retardant, inhibits the activity by 55% Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
geranylgeranyl diphosphate pH 9.0, 30°C Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Ricinus communis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highly dependent on, maximal stimulation of the activity above 5 mM, Mn2+ is much less effective Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
native enzyme, gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Ricinus communis regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite casbene + diphosphate diterpene phytoalexin, product of a single enzyme-catalyzed transformation ?

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
Hale variety, N5 variety
-
Ricinus communis
-
castor bean, unknown variety
-

Purification (Commentary)

Purification (Comment) Organism
partial Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
seedling infected with the fungus Rhizpus stolonifer, no detectable levels of casbene synthetase mRNA in healthy seedlings Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0437
-
-
Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
Ricinus communis casbene + diphosphate
-
?
geranylgeranyl diphosphate regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite Ricinus communis casbene + diphosphate diterpene phytoalexin, product of a single enzyme-catalyzed transformation ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9 half-maximal activity at pH 6.0 and 9.8 Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
6 9.8 half-maximal activity at pH 6.0 and 9.8 Ricinus communis