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Literature summary for 4.2.3.77 extracted from

  • Nieuwenhuizen, N.J.; Wang, M.Y.; Matich, A.J.; Green, S.A.; Chen, X.; Yauk, Y.-K.; Beuning, L.L.; Atkinson, R.G.
    Two terpene synthases are responsible for the major sesquiterpenes emitted from the flowers of kiwifruit (Actinidia deliciosa) (2009), J. Exp. Bot., 60, 3203-3219.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.77

Cloned(Commentary)

Cloned (Comment) Organism
Agrobacterium tumefaciens-mediated expression in Nicotiana benthamiana Actinidia deliciosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, presence of Mg2+ Actinidia deliciosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Actinidia deliciosa 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ preferred over Mn2+ Actinidia deliciosa
Mn2+ 43% of the maximal velocity in presence of Mg2+ Actinidia deliciosa
additional information divalent cation required Actinidia deliciosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65200
-
 x * 65200, calculated Actinidia deliciosa

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa Q32W37
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower expression is significantly higher in flowers than in leaf tissue. Expression increases at 08.00 h and then drops to about a half by 20.00 h Actinidia deliciosa
-
leaf expression is significantly higher in flowers than in leaf tissue Actinidia deliciosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Actinidia deliciosa (+)-germacrene D + diphosphate single product, exclusively synthesizes the (+)-configuration of germacrene D ?

Subunits

Subunits Comment Organism
? x * 65200, calculated Actinidia deliciosa

Synonyms

Synonyms Comment Organism
GDS1
-
 Actinidia deliciosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, presence of Mg2+ Actinidia deliciosa

pI Value

Organism Comment pI Value Maximum pI Value
Actinidia deliciosa calculated
-
 6.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.7
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, presence of Mg2+ Actinidia deliciosa