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Literature summary for 4.2.3.77 extracted from
- Cloning, expression, purification and characterization of recombinant (+)-germacrene D synthase from Zingiber officinale (2006), Arch. Biochem. Biophys., 452, 17-28.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Zingiber officinale |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 33% of the activity with Mg2+ | Zingiber officinale |  |
| Mg2+ | maximum activity at 0.1 mM | Zingiber officinale | |
| Mn2+ | 0.1 mM, 24% of the activity with Mg2+ | Zingiber officinale | |
| additional information | divalent cation absolutely required, inactive with Cu2+ or Zn2+ | Zingiber officinale | |
| Ni2+ | 0.5 mM, 6% of the activity with Mg2+ | Zingiber officinale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 63800, calculated, x * 60000, SDS-PAGE | Zingiber officinale |
| 63800 | - |
x * 63800, calculated, x * 60000, SDS-PAGE | Zingiber officinale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zingiber officinale | Q0VHD6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Zingiber officinale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Zingiber officinale | (+)-germacrene D + diphosphate | products are 50.2% (+)-germacrene D, 17.1% germacrene B, plus a number of minor products | ? | |
| additional information | no substrate: geranyl diphosphate | Zingiber officinale | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63800, calculated, x * 60000, SDS-PAGE | Zingiber officinale |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDS | - |
Zingiber officinale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Zingiber officinale |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | almost completely inactive below and above | Zingiber officinale |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Zingiber officinale | calculated | - |
5.6 |
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