Literature summary for 4.2.3.72 extracted from

  • Schmidt, C.O.; Bouwmeester, H.J.; Buelow, N.; Koenig, W.A.
    Isolation, characterization, and mechanistic studies of (-)-alpha-gurjunene synthase from Solidago canadensis (1999), Arch. Biochem. Biophys., 364, 167-177.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0055
-
(2E,6E)-farnesyl diphosphate pH 7.0, 30°C Solidago canadensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Solidago canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
1 * 60000, SDS-PAGE Solidago canadensis
48000
-
gel filtration Solidago canadensis

Organism

Organism UniProt Comment Textmining
Solidago canadensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Solidago canadensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49.9
-
pH 7.0, 30°C Solidago canadensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate initial step is the formation of a germacrenyl cation after abstraction of the diphosphate group. Internal shifts of electron pairs lead to the patchoulenyl cation, which then undergoes a double 1,2-hydride shift. Rearrangement leads to the aromadendrenyl cation, which then undergoes a 1,2-hydride shift. Abstraction of a proton by the enzyme yields isoledene, which is reprotonated at the active site of the enzyme. At this junction two proton abstractions are possible, one leading to (-)-alpha-gurjunene and the other to (+)-gamma-gurjunene Solidago canadensis (-)-alpha-gurjunene + diphosphate product ratio 91% (-)-alpha-gurjunene and 9% (+)-gamma-gurjunene ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Solidago canadensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
presence of Mg2+ Solidago canadensis