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Literature summary for 4.2.3.70 extracted from

  • Frister, T.; Hartwig, S.; Alemdar, S.; Schnatz, K.; Thoens, L.; Scheper, T.; Beutel, S.
    Characterisation of a recombinant patchoulol synthase variant for biocatalytic production of terpenes (2015), Appl. Biochem. Biotechnol., 176, 2185-2201 .
    View publication on PubMed
Search for more literature for 4.2.3.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pogostemon cablin

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 (2Z,6E)-farnesyl diphosphate at pH 7.3 and 38°C Pogostemon cablin

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pogostemon cablin
additional information no activity with Ca2+, Ba2+, Mn2+, Ni2+, Co2+and Cu2+ Pogostemon cablin

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6Z)-farnesyl diphosphate Pogostemon cablin
-
 patchoulol + diphosphate
-
 ?
(2Z,6E)-farnesyl diphosphate Pogostemon cablin
-
 patchoulol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pogostemon cablin Q49SP3 Indian Patchouli
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Pogostemon cablin

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6Z)-farnesyl diphosphate
-
 Pogostemon cablin patchoulol + diphosphate
-
 ?
(2Z,6E)-farnesyl diphosphate
-
 Pogostemon cablin patchoulol + diphosphate
-
 ?
Geranyl diphosphate
-
 Pogostemon cablin ?
-
 ?
additional information no activity with geranylgeranyl diphosphate Pogostemon cablin ?
-
 ?
neryl diphosphate
-
 Pogostemon cablin ?
-
 ?

Synonyms

Synonyms Comment Organism
PTS variant 1 Pogostemon cablin

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Pogostemon cablin

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.072
-
 (2Z,6E)-farnesyl diphosphate at pH 7.3 and 38°C Pogostemon cablin

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Pogostemon cablin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 (2Z,6E)-farnesyl diphosphate at pH 7.3 and 38°C Pogostemon cablin