Application | Comment | Organism |
---|---|---|
synthesis | fusion to thioredoxin and careful codon optimization of the eukaryotic sequence improves solubl expression on average by 42% in comparison to an unoptimized, His-tagged construct | Pogostemon cablin |
Cloned (Comment) | Organism |
---|---|
expression in Eschrichia coli | Pogostemon cablin |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fusion to thioredoxin and careful codon optimization of the eukaryotic sequence improves solubl expression on average by 42% in comparison to an unoptimized, His-tagged construct | Pogostemon cablin |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pogostemon cablin | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required | Pogostemon cablin |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pogostemon cablin | Q49SP3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | - |
Pogostemon cablin | patchoulol + diphosphate | main product of recombinant enzyme is germacrene A, additional formation of + alpha-bulnesene | ? |