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Literature summary for 4.2.3.7 extracted from
- Partial purification and characterization of pentalenene synthase (1987), Arch. Biochem. Biophys., 254, 421-429.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | plus pentalene: no inhibition alone, both products bind cooperatively at the active site | Streptomyces sp. |  |
| Mn2+ | divalent metal required, Mg2+ or Mn2+, inhibition above 2.5 mM | Streptomyces sp. | |
| Pentalenene plus diphosphate | no inhibition alone, both products bind cooperatively at the active site | Streptomyces sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00077 | - |
(E,E)-farnesyl diphosphate | 30°C, pH 8.4 | Streptomyces sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent metal required, Mg2+ or Mn2+ | Streptomyces sp. | |
| Mn2+ | divalent metal required, Mg2+ or Mn2+, inhibition above 2.5 mM | Streptomyces sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
- |
Streptomyces sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| farnesyl diphosphate | Streptomyces sp. | - |
pentalenene + diphosphate | - |
? | |
| farnesyl diphosphate | Streptomyces sp. UC5319 | - |
pentalenene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | - |
UC5319 | - |
| Streptomyces sp. UC5319 | - |
UC5319 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Streptomyces sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.287 | - |
30°C, pH 8.4 | Streptomyces sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crude enzyme stable for up to 6 days | Streptomyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans,6-trans-farnesyl diphosphate | - |
Streptomyces sp. | pentalenene + diphosphate | - |
? | |
| 2-trans,6-trans-farnesyl diphosphate | - |
Streptomyces sp. UC5319 | pentalenene + diphosphate | - |
? | |
| farnesyl diphosphate | - |
Streptomyces sp. | pentalenene + diphosphate | - |
? | |
| farnesyl diphosphate | trans,trans | Streptomyces sp. | pentalenene + diphosphate | - |
? | |
| farnesyl diphosphate | - |
Streptomyces sp. UC5319 | pentalenene + diphosphate | - |
? | |
| farnesyl diphosphate | trans,trans | Streptomyces sp. UC5319 | pentalenene + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Streptomyces sp. |
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