Application | Comment | Organism |
---|---|---|
analysis | method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host provides for direct functional testing of the encoded gene product | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
expression in Petunia hybrida infiltrated with Agrobacterium tumefaciens harboring the enzyme gene | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q7X9A3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Nicotiana tabacum | 5-epi-aristolochene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
g110 | - |
Nicotiana tabacum |