Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21DE3 cells | Fusarium sporotrichioides |
Crystallization (Comment) | Organism |
---|---|
the structures of the mutants are solved at resolutions ranging from 2.1 to 2.7 A | Fusarium sporotrichioides |
Protein Variants | Comment | Organism |
---|---|---|
N225D | mutation in the fungal NSE motif, responsible for chelating Mg2+, mutant generates a different distribution of sesquiterpene products | Fusarium sporotrichioides |
N225D, S229T | mutation in the fungal NSE motif, responsible for chelating Mg2+, inactive mutant | Fusarium sporotrichioides |
S229T | mutation in the fungal NSE motif, responsible for chelating Mg2+, mutant generates a different distribution of sesquiterpene products | Fusarium sporotrichioides |
Y295F | mutation in the fungal NSE motif, responsible for chelating Mg2+, mutant generates a different distribution of sesquiterpene products | Fusarium sporotrichioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000069 | - |
farnesyl diphosphate | mutant Y295F | Fusarium sporotrichioides | |
0.000078 | - |
farnesyl diphosphate | wild-type | Fusarium sporotrichioides | |
0.000485 | - |
farnesyl diphosphate | mutant N225D | Fusarium sporotrichioides | |
0.006 | - |
farnesyl diphosphate | mutant S229T | Fusarium sporotrichioides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Fusarium sporotrichioides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
- |
Fusarium sporotrichioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans,trans-farnesyl diphosphate | Fusarium sporotrichioides | - |
trichodiene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium sporotrichioides | P13513 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Fusarium sporotrichioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans,trans-farnesyl diphosphate | - |
Fusarium sporotrichioides | trichodiene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Fusarium sporotrichioides |
Synonyms | Comment | Organism |
---|---|---|
trichodiene synthase | - |
Fusarium sporotrichioides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Fusarium sporotrichioides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
farnesyl diphosphate | mutant N225D | Fusarium sporotrichioides | |
0.015 | - |
farnesyl diphosphate | mutant S229T | Fusarium sporotrichioides | |
0.111 | - |
farnesyl diphosphate | mutant Y295F | Fusarium sporotrichioides | |
0.138 | - |
farnesyl diphosphate | wild-type | Fusarium sporotrichioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
activity assay | Fusarium sporotrichioides |