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Literature summary for 4.2.3.6 extracted from

  • Cane, D.E.; Yang, G.
    Trichodiene syntase. Stereochemical studies of the cryptic allylic diphosphate isomerase activity using an anomalous substrate (1994), J. Org. Chem., 59, 5794-5798.
No PubMed abstract available
Search for more literature for 4.2.3.6

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate Escherichia coli
-
 trichodiene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate Fusarium sporotrichioides
-
 trichodiene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 BL21 DE3 /pZW03
-
Fusarium sporotrichioides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
BL21 DE3/pZW03 Escherichia coli
BL21 DE3/pZW03 Fusarium sporotrichioides

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate mechanism Fusarium sporotrichioides
a
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate stereochemistry, mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.222
-
-
 Escherichia coli
0.222
-
-
 Fusarium sporotrichioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7S)-6,7-dihydrofarnesyl diphosphate
-
 Escherichia coli dihydrofarnesene isomers + (E)-6,7-dihydrofarnesol + (3S,7S)-6,7-dihydronerolidol
-
 ?
(7S)-6,7-dihydrofarnesyl diphosphate
-
 Fusarium sporotrichioides dihydrofarnesene isomers + (E)-6,7-dihydrofarnesol + (3S,7S)-6,7-dihydronerolidol
-
 ?
(E,E)-farnesyl diphosphate
-
 Escherichia coli trichodiene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate
-
 Fusarium sporotrichioides trichodiene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate formation of trichodiene involves initial generation of (3R)-nerolidyl diphosphate Escherichia coli trichodiene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate formation of trichodiene involves initial generation of (3R)-nerolidyl diphosphate Fusarium sporotrichioides trichodiene + diphosphate
-
 ?
additional information reaction proceeds via isomerization of farnesyl diphosphate to (3R)-nerolidyl diphosphate and further to trichodiene Escherichia coli ?
-
 ?