Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.57 extracted from

  • Yang, J.; Li, Z.; Guo, L.; Du, J.; Bae, H.
    Biosynthesis of beta-caryophyllene, a novel terpene-based high-density biofuel precursor, using engineered Escherichia coli (2016), Renewable Energy, 99, 216-223 .
No PubMed abstract available
Search for more literature for 4.2.3.57

Application

Application Comment Organism
biofuel production acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce b-caryophyllene from reproducible sources. Contribution by recombinant production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain Arabidopsis thaliana
biofuel production acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce b-caryophyllene from reproducible sources. Contribution by recombinant production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain Artemisia annua

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized QHS1 gene, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH Artemisia annua
expressed in Escherichia coli strain YJM59 Arabidopsis thaliana
expressed in Escherichia coli strain YJM59 Artemisia annua
expressed in Escherichia coli strain YJM59 Zea perennis
gene TPS21, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1-deoxy-D-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways are employed. Geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene), and beta-caryophyllene synthase genes are co-overexpressed in the engineered strain. The final genetically modified strain, YJM59, produces 220 mg/l of beta-caryophyllene in flask culture. Evaluation of fed-batch fermentation for the production of beta-caryophyllene. After induction for 60 h, the YJM59 strain produces beta-caryophyllene at a concentration of 1520 mg/l. The volumetric production fermented in the aerobic fed-batch is 0.34 mg/(l/h/OD600) and the conversion efficiency of glucose to beta-caryophyllene (gram to gram) is 1.69%. Method evaluation with beta-caryophyllene synthases from different origins, QHS1 from Artemisia annua is the most effective of the three enzymes, compared to TPS21 from Arabidopsis thaliana and TPS23 from Zea perennis. Substrate channeling Arabidopsis thaliana
additional information production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1-deoxy-D-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways are employed. Geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene), and beta-caryophyllene synthase genes are co-overexpressed in the engineered strain. The final genetically modified strain, YJM59, produces 220 mg/l of beta-caryophyllene in flask culture. Evaluation of fed-batch fermentation for the production of beta-caryophyllene. After induction for 60 h, the YJM59 strain produces beta-caryophyllene at a concentration of 1520 mg/l. The volumetric production fermented in the aerobic fed-batch is 0.34 mg/(l/h/OD600) and the conversion efficiency of glucose to beta-caryophyllene (gram to gram) is 1.69%. Method evaluation with beta-caryophyllene synthases from different origins, QHS1 from Artemisia annua is the most effective of the three enzymes, compared to TPS21 from Arabidopsis thaliana and TPS23 from Zea perennis. Substrate channeling Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Arabidopsis thaliana
-
 (-)-beta-caryophyllene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Artemisia annua
-
 (-)-beta-caryophyllene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Zea perennis
-
 (-)-beta-caryophyllene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q84UU4
-
-
Artemisia annua Q8SA63
-
-
Zea perennis B2C4D8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Arabidopsis thaliana (-)-beta-caryophyllene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Artemisia annua (-)-beta-caryophyllene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Zea perennis (-)-beta-caryophyllene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
alpha-humulene/(-)-(E)-beta-caryophyllene synthase UniProt Arabidopsis thaliana
QHS1
-
 Artemisia annua
TPS21
-
 Arabidopsis thaliana
tps23
-
 Zea perennis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay at Arabidopsis thaliana
30
-
 in vivo assay at Artemisia annua

General Information

General Information Comment Organism
metabolism beta-caryophyllene is produced via the mevalonate (MVA)-mediated pathway, overview Arabidopsis thaliana
metabolism beta-caryophyllene is produced via the mevalonate (MVA)-mediated pathway, overview Artemisia annua