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Literature summary for 4.2.3.56 extracted from

  • Steele, C.L.; Crock, J.; Bohlmann, J.; Croteau, R.
    Sesquiterpene synthases from grand fir (Abies grandis). Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of delta-selinene synthase and gamma-humulene synthase (1998), J. Biol. Chem., 273, 2078-2089.
    View publication on PubMed
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ gamma-humulene synthase can utilize Mg2+ or Mn2+ with comparable velocities in the cyclization reaction Abies grandis
Mn2+ gamma-humulene synthase can utilize Mg2+ or Mn2+ with comparable velocities in the cyclization reaction Abies grandis
additional information does not utilize K+ in the cyclization reaction Abies grandis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67940
-
 calculated from amino acid sequence Abies grandis

Organism

Organism UniProt Comment Textmining
Abies grandis O64405 grand fir
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Source Tissue

Source Tissue Comment Organism Textmining
seed
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 Abies grandis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
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 Abies grandis gamma-humulene + diphosphate main product with 28.6% yield ?
additional information gamma-humulene synthase produces mainly gamma-humulene (28.6% yield) from (2E,6E)-farnesyl diphosphate, further products are sibirene (15.1% yield), longifolene (11.8% yield), beta-himachalene (7.2% yield), alpha-himachalene (4.8% yield), beta-bisabolene (3.9% yield), alpha-longipinene (3.4% yield), sativene (3.1% yield), alpha-ylangene (2.7% yield), beta-gurjunene (2.0% yield), gamma-bisabolene (1.9% yield), beta-longipinene (1.5% yield), (E)-beta-farnesene (1.3% yield), (E)-alpha-bisabolene (0.9% yield), delta-amorphene and alpha-amorphene (0.5% yield each), beta-ylangene (0.3% yield), 1,5,9-trimethylcyclododeca-1,5,9-triene and longicyclene (0.2% yield each), as well as beta-cubebene, alpha-copaene, alpha-cubebene, (Z,E)-germacrene B, and cyclosativene (0.1% yield each), along with 26 unknown sesquiterpene olefins (4.1% total yield) Abies grandis ?
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 ?

Synonyms

Synonyms Comment Organism
gamma-humulene synthase
-
 Abies grandis