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Literature summary for 4.2.3.55 extracted from
- Functional characterization of novel sesquiterpene synthases from indian sandalwood, Santalum album (2015), Sci. Rep., 5, 10095 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from wood shavings from the interface of heartwood and sapwood from mature sandalwood trees, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative real-time enzyme expression analysis, and transcriptome screening, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) | Santalum album |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Santalum album | |
| 0.0126 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 30°C, recombinant His6-tagged enzyme | Santalum album |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | enzyme SaBS lacks an N-terminal organelle targeting sequence, suggesting that the enzyme is directed to the cytoplasm | Santalum album | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | Santalum album | - |
(S)-beta-bisabolene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Santalum album | A0A0A0RDZ8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography to homogeneity | Santalum album |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Santalum album | (S)-beta-bisabolene + diphosphate | - |
? | |
| additional information | product identification by GC-MS analysis | Santalum album | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65900, about, sequence calculation | Santalum album |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bisabolene synthase | - |
Santalum album |
| SaBS | - |
Santalum album |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Santalum album |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00067 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 30°C, recombinant His6-tagged enzyme | Santalum album | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Santalum album |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Santalum album | sequence calculation | - |
5.48 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 30°C, recombinant His6-tagged enzyme | Santalum album | |
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