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Literature summary for 4.2.3.52 extracted from
- Cloning and functional characterization of beta-phellandrene synthase from Lavandula angustifolia (2011), Planta, 233, 685-696.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lavandula angustifolia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00655 | - |
geranyl diphosphate | pH 6.5, 30°C | Lavandula angustifolia |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Lavandula angustifolia | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62300 | - |
x * 62300, calculated, including transit peptide, x * 62300, recombinant protein including His-Tag, SDS-PAGE | Lavandula angustifolia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lavandula angustifolia | E9N3U9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | very poor expression | Lavandula angustifolia | - |
| leaf | strong expression in young leaf | Lavandula angustifolia | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | enzyme displays a clear preference for geranyl diphosphate over neryl diphosphate | Lavandula angustifolia | (4S)-beta-phellandrene + diphosphate | in addition, enzyme produces small quantities of limonene | ? | |
| additional information | no substrate: farnesyl diphosphate | Lavandula angustifolia | ? | - |
? | |
| neryl diphosphate | enzyme displays a clear preference for geranyl diphosphate over neryl diphosphate | Lavandula angustifolia | beta-phellandrene + diphosphate | in addition, enzyme produces small quantities of limonene | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62300, calculated, including transit peptide, x * 62300, recombinant protein including His-Tag, SDS-PAGE | Lavandula angustifolia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-phellandrene synthase | - |
Lavandula angustifolia |
| OPHLS | - |
Lavandula angustifolia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lavandula angustifolia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
geranyl diphosphate | pH 6.5, 30°C | Lavandula angustifolia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Lavandula angustifolia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.83 | - |
geranyl diphosphate | pH 6.5, 30°C | Lavandula angustifolia | |
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