Literature summary for 4.2.3.52 extracted from

Bohlmann, J.; Phillips, M.; Ramachandiran, V.; Katoh, S.; Croteau, R.
cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis) (1999), Arch. Biochem. Biophys., 368, 232-243.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Abies grandis

Organism

Organism	UniProt	Comment	Textmining
Abies grandis	Q9M7D1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	soluble enzyme preparations from Escherichia coli BL21(DE3)/pSAG8(M), expressing (-)-beta-phellandrene synthase convert geranyl diphosphate to four products. beta-phellandrene (52%) as the major olefin, and lesser amounts of (1S,5S)-beta-pinene (34%), (1S,5S)-apinene (8.5%), and (4S)-limonene (6%) are identified. The stereochemistry of beta-phellandrene is not confirmed directly. Nevertheless, stereochemical considerations based on the established absolute configuration of the coproducts, and the natural occurrence of (4S)-beta-phellandrene in the turpentine, suggests that the biosynthetic product is the mechanistically related (4S)-antipode derived via the (3S)-linalyl diphosphate intermediate. The product of the ag8 gene is therefore designated (4S)-beta-phellandrene synthase	Abies grandis	(4S)-beta-phellandrene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
(-)-(4S)-beta-phellandrene synthase	-	Abies grandis
(-)-beta-phellandrene synthase	-	Abies grandis

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Release 2023.1 (January 2023)