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Literature summary for 4.2.3.49 extracted from

  • Schnee, C.; Köllner, T.G.; Gershenzon, J.; Degenhardt, J.
    The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-beta-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damage (2002), Plant Physiol., 130, 2049-2060.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.49

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 (2E,6E)-farnesyl diphosphate 30°C Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal ion cofactor is required for enzyme activity, KM-value for Mg2+: 0.47 mM. Mg2+ and Mn2+ give substantial activity, with Mg2+ at 5 mM giving the highest activity Zea mays
Mg2+ divalent metal ion cofactor is required for enzyme activity, KM-value for Mn2+: 0.026 mM. Mg2+ and Mn2+ give substantial activity, with Mg2+ at 5 mM giving the highest activity Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67400
-
 1 * 67400, calculated from sequence Zea mays
67400
-
 2 * 67400, calculated from sequence Zea mays
71000
-
 monomer, gel filtration Zea mays
140000
-
 dimer, recombinant enzyme, gel filtration Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O Zea mays herbivore-induced production of (3R,6E)-nerolidol, an intermediate in the formation of 4,8-dimethyl-1,3(E),7-nonatriene (3R,6E)-nerolidol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays Q84ZW8 cultivar B73 and Delprim
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars. 16 hours after the initiation of herbivore damage, there is a significant elevation in transcript level Zea mays
-

Storage Stability

Storage Stability Organism
-80°C, stable for at least 1 week Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O herbivore-induced production of (3R,6E)-nerolidol, an intermediate in the formation of 4,8-dimethyl-1,3(E),7-nonatriene Zea mays (3R,6E)-nerolidol + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O in the presence of geranyl diphosphate the enzyme catalyzes the formation of (3R)-linalool and geraniol Zea mays (3R,6E)-nerolidol + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 67400, calculated from sequence Zea mays
monomer 1 * 67400, calculated from sequence Zea mays

Synonyms

Synonyms Comment Organism
terpene synthase 1
-
 Zea mays
TPS1
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
6 7.7 half-maximal activity at pH 6.0 and pH 7.7 Zea mays

Expression

Organism Comment Expression
Zea mays transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars up