Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Malus domestica |
Protein Variants | Comment | Organism |
---|---|---|
D326A | alpha-farnesene synthase, monoterpene synthase and prenyltransferase activities are lost in the mutant | Malus domestica |
D326A/D330A | alpha-farnesene synthase, monoterpene synthase and prenyltransferase activities are lost in the mutant | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Na2MoO4 | 10 mM, 96% inhibition | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 30-50 mM, enhances activity 5fold. Km: 3 mM. Addition of K+ reduces monoterpene synthase activity | Malus domestica | |
Mg2+ | Km: 0.7 mM | Malus domestica | |
Mn2+ | Km: 0.015 mM | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Malus domestica | - |
(3E,6E)-alpha-farnesene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | Q84LB2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit skin | - |
Malus domestica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
- |
Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Malus domestica | (3E,6E)-alpha-farnesene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | when farnesyl diphosphate, synthesised from 96% (E,E)-farnesol, is incubated with recombinant protein, (E,E)- and (Z,E)-alpha-farnesene are produced in a ratio of 96:4, respectively | Malus domestica | (3E,6E)-alpha-farnesene + beta-farnesene + diphosphate | - |
? | |
geranyl diphosphate | at 18% of the optimised rate for alpha-farnesene synthesis from farnesyl diphosphate | Malus domestica | linalool + (Z)-beta-ocimene + (E)-beta-ocimene + beta-myrcene | - |
? | |
additional information | although (E,E)-farnesyl diphosphate is the preferred substrate, the enzyme accepts all four isomeric forms of the farnesyl diphosphate precursor. Both isomers of beta-farnesene are also synthesised by the enzyme presumably from a specific farnesene isomer. The enzyme also produces alpha-farnesene by a reaction involving coupling of geranyl diphosphate and isoprenyl diphosphate but at less than 1% of the rate with farnesyl diphosphate | Malus domestica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Malus domestica |
Synonyms | Comment | Organism |
---|---|---|
alpha-farnesene synthase | - |
Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Malus domestica |