Any feedback?
Literature summary for 4.2.3.43 extracted from
- Structure and function of fusicoccadiene synthase, a hexameric bifunctional diterpene synthase (2016), ACS Chem. Biol., 11, 889-899 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)RIL-CodonPlus cells | Diaporthe amygdali |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method | Diaporthe amygdali |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D474A | partially inactivated mutant | Diaporthe amygdali |
| D92A | the mutant enzxme does not generate any cyclic products yet retains geranylgeranyl diphosphate synthase activity | Diaporthe amygdali |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00014 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali |  |
| 0.00063 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
| 0.0092 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | essential | Diaporthe amygdali | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 502000 | - |
gel filtration | Diaporthe amygdali |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate | Diaporthe amygdali | - |
fusicocca-2,10(14)-diene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diaporthe amygdali | A2PZA5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Diaporthe amygdali |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Diaporthe amygdali | (E,E)-farnesol + (E)-nerolidol + farnesene + diphosphate | - |
? | |
| geranyl diphosphate | - |
Diaporthe amygdali | geraniol + linalool + diphosphate | - |
? | |
| geranylgeranyl diphosphate | - |
Diaporthe amygdali | fusicocca-2,10(14)-diene + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | small-angle X-ray scattering | Diaporthe amygdali |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fusicoccadiene synthase | - |
Diaporthe amygdali |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
| 0.027 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
| 0.036 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
| 33 | - |
geranylgeranyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
| 190 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Diaporthe amygdali | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
