Literature summary for 4.2.3.40 extracted from

Ro, D.K.; Ehlting, J.; Keeling, C.I.; Lin, R.; Mattheus, N.; Bohlmann, J.
Microarray expression profiling and functional characterization of AtTPS genes: duplicated Arabidopsis thaliana sesquiterpene synthase genes At4g13280 and At4g13300 encode root-specific and wound-inducible (Z)-gamma-bisabolene synthases (2006), Arch. Biochem. Biophys., 448, 104-116.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	wound-inducible (Z)-gamma-bisabolene synthase, mechanical wounding of leaves induces local expression of At4g13280 and At4g13300	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
root-specific genes At4g13280 and At4g13300 show constitutive promoter activities in the cortex and sub-epidermal layers of Arabidopsis thaliana roots, hierarchical cluster analysis of terpene synthases TPS gene expression, expression analysis, microarray profiling, overview. Expression in Escherichia coli strain BL21	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	Arabidopsis thaliana	-	(Z)-gamma-bisabolene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	ecotype Ler, root-specific genes At4g13280 and At4g13300 encoding isozymes TPS12 and TPS13	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	young rosette leaves, enzyme induction by mechanical wounding	Arabidopsis thaliana	-
additional information	enzyme tissue localization study	Arabidopsis thaliana	-
root	root-specific (Z)-gamma-bisabolene synthase, in cortex and sub-epidermal layers	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Arabidopsis thaliana	(Z)-gamma-bisabolene + diphosphate	-	?
(2E,6E)-farnesyl diphosphate	product identification by GC-MS, additional minor products are E-nerolidol and alpha-bisabolol, overview	Arabidopsis thaliana	(Z)-gamma-bisabolene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
TPS12	-	Arabidopsis thaliana
TPS13	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	assay at	Arabidopsis thaliana

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Release 2023.1 (January 2023)