Literature summary for 4.2.3.4 extracted from

Mittelstaedt, G.; Negron, L.; Schofield, L.R.; Marsh, K.; Parker, E.J.
Biochemical and structural characterisation of dehydroquinate synthase from the New Zealand kiwifruit Actinidia chinensis (2013), Arch. Biochem. Biophys., 537, 185-191.

Search for more literature for 4.2.3.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Actinidia chinensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular replacement based on the structure of Aspergillus nidulans enzyme, PDB code 1NR5, revealing a homodimeric protein. Each monomer has a NAD+ binding site nestled between the distinct N- and C-terminal domains	Actinidia chinensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.3	-	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate	pH 7.5, 25°C	Actinidia chinensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	-	Actinidia chinensis
Fe2+	110% of the activity in presence of Co2+	Actinidia chinensis
additional information	a divalent metal ion is required	Actinidia chinensis
Zn2+	25% of the activity in presence of Co2+	Actinidia chinensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41097	-	2 * 41097, mass spectrometry, 2 * 41098, calculated	Actinidia chinensis
41098	-	2 * 41097, mass spectrometry, 2 * 41098, calculated	Actinidia chinensis
79200	-	light scattering	Actinidia chinensis
81000	-	gel filtration	Actinidia chinensis

Organism

Organism	UniProt	Comment	Textmining
Actinidia chinensis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate	-	Actinidia chinensis	3-dehydroquinate + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 41097, mass spectrometry, 2 * 41098, calculated	Actinidia chinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
41	-	melting temperature, in presence of either Zn2+ or Co2+	Actinidia chinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20.1	-	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate	pH 7.5, 25°C	Actinidia chinensis

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no cofactor: NADP+	Actinidia chinensis
NAD+	required	Actinidia chinensis

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Release 2023.1 (January 2023)