Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli Rosetta DE3. The enzyme is insoluble when expressed in Escherichia coli but is partially solubilised when KCl is included in the cell lysis buffer | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inactivates the enzyme, and enzyme activity is restored by several divalent metal ions including (in order of decreasing effectiveness) Cd2+, Co2+, Zn2+, and Mn2+ | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | pH 6.8, 60°C | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates | Pyrococcus furiosus | |
Co2+ | activates | Pyrococcus furiosus | |
Mn2+ | activates | Pyrococcus furiosus | |
Zn2+ | activates | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37397 | - |
2 * 37397, electrospray ionisation mass spectrometry | Pyrococcus furiosus |
72000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | Pyrococcus furiosus | the enzyme catalyses the second step of the shikimate pathway to aromatic compounds | 3-dehydroquinate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U0A8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Pyrococcus furiosus | 3-dehydroquinate + phosphate | - |
? | |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | the enzyme catalyses the second step of the shikimate pathway to aromatic compounds | Pyrococcus furiosus | 3-dehydroquinate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37397, electrospray ionisation mass spectrometry | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
DHQS | - |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
active up to | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
Tm-value | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | pH 6.8, 60°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 9.5 | pH 6.3: 65% of maximal activity, pH 9.5: about 65% of maximal activity | Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyses the second step of the shikimate pathway to aromatic compounds | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
811 | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | pH 6.8, 60°C | Pyrococcus furiosus |