Application | Comment | Organism |
---|---|---|
medicine | the enzymes of the shikimate pathway are attractive drug targets for the treatment of tuberculosis | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
into the pET23a+ vector for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Mycobacterium tuberculosis | |
Zn2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38100 | - |
determined by SDS-PAGE | Mycobacterium tuberculosis |
38140 | - |
determined by ESI-MS | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | Mycobacterium tuberculosis | - |
3-dehydroquinate + phosphate | - |
ir | |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | Mycobacterium tuberculosis H37Rv | - |
3-dehydroquinate + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPX9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPX9 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Q-Sepharose Fast Flow, a HiLoad 16/10 phenyl-Sepharose, and a Sephacryl S-200 HR column | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Mycobacterium tuberculosis | 3-dehydroquinate + phosphate | - |
ir | |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Mycobacterium tuberculosis H37Rv | 3-dehydroquinate + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
3-dehydroquinate synthase | - |
Mycobacterium tuberculosis |
dehydroquinate synthase | - |
Mycobacterium tuberculosis |
DHQ synthase | - |
Mycobacterium tuberculosis |
DHQS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
activity assay | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |