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Literature summary for 4.2.3.39 extracted from
- Cloning, expression, and characterization of epi-cedrol synthase, a sesquiterpene cyclase from Artemisia annua L (1999), Arch. Biochem. Biophys., 369, 213-222.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from a cDNA library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue | Artemisia annua |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | highly activating at 0.060 mM, inhibiting at 0.12 mM | Artemisia annua |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 25°C, recombinant enzyme | Artemisia annua | |
| 0.0013 | - |
(2E,6E)-farnesyl diphosphate | pH 9.0, 25°C, recombinant enzyme | Artemisia annua | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates at 2 mM | Artemisia annua | |
| Mn2+ | highly activating at 0.060 mM, inhibiting at 0.12 mM | Artemisia annua | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63500 | - |
x * 63500, about, sequence calculation | Artemisia annua |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | Artemisia annua | the enzyme is involved in the artemisinin biosynthesis pathway, overview | 8-epi-cedrol + diphosphate | - |
? | |
| additional information | Artemisia annua | Sesquiterpene cyclases or synthases catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemisia annua | Q9LLR9 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O = 8-epi-cedrol + diphosphate | reaction mechanism, overview | Artemisia annua |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Artemisia annua | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | the enzyme is involved in the artemisinin biosynthesis pathway, overview | Artemisia annua | 8-epi-cedrol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | the native enzyme is specific for farnesyl diphosphate as substrate | Artemisia annua | 8-epi-cedrol + diphosphate | - |
? | |
| geranyl diphosphate + H2O | geranyl diphosphate is converted to monoterpenes by the recombinant enzyme at a rate of about 15% of that observed with farnesyl diphosphate as substrate | Artemisia annua | ? + diphosphate | - |
? | |
| additional information | Sesquiterpene cyclases or synthases catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types | Artemisia annua | ? | - |
? | |
| additional information | product formation specificity, overview. Native epi-cedrol synthase is not active with geranylgeranyl diphosphate as substrate. The recombinant enzyme catalyzes the formation of both olefinic sesquiterpene, i.e. 57% alpha-cedrene, 13% beta-cedrene, 5% (E)-beta-farnesene , 1% alpha-acoradiene, 8% (E)-alpha-bisabolene, and 16% of three unknown olefins, but mainly oxygenates sesquiterpenes, 97% of total sesquiterpene generated, composed of 96% epi-cedrol and 4% cedrol, from farnesyl diphosphate, GC-MS product analysis, overview | Artemisia annua | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63500, about, sequence calculation | Artemisia annua |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sesquiterpene cyclase | - |
Artemisia annua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Artemisia annua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | alcohol product formation | Artemisia annua |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9 | - |
Artemisia annua |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Artemisia annua | sequence calculation | - |
4.94 |
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