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Literature summary for 4.2.3.36 extracted from

  • Nakano, C.; Hoshino, T.; Sato, T.; Toyomasu, T.; Dairi, T.; Sassa, T.
    Substrate specificity of the CYC2 enzyme from Kitasatospora griseola: production of sclarene, biformene and novel bicyclic diterpenes by the enzymatic reactions of labdane- and halimane-type diterpene diphosphates (2010), Tetrahedron Lett., 51, 125-128.
No PubMed abstract available
Search for more literature for 4.2.3.36

Application

Application Comment Organism
synthesis CYC2 is a promising tool for introducing a buta-1,3-diene moiety into molecules by the enzymatic reactions Kitasatospora griseola

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ presence is essential for enzymic activity Kitasatospora griseola
Mg2+ the divalent metal ion is essential for the enzyme activities of all diterpene cyclases including the dephosphorylating CYC2 enzyme. Therefore, the CYC2 catalyzes Type A-like reaction, that is, the diphosphate-removal reaction followed by the formation of allylic carbocation Kitasatospora griseola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Kitasatospora griseola CYC2 is not a cyclase, it shows broad substrate specificity. All of the three bicyclic diterpenes labdane, halimane, and clerodane, occuring in nature, are accepted as substrates of the CYC2, reactivity in a coupled assay with ent-CDP synthase, EC 5.5.1.13, overview ?
-
 ?
terpentedienyl diphosphate Kitasatospora griseola
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 terpentetriene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Kitasatospora griseola
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-
-
Kitasatospora griseola Q9AJE3
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CYC2 is not a cyclase, it shows broad substrate specificity. All of the three bicyclic diterpenes labdane, halimane, and clerodane, occuring in nature, are accepted as substrates of the CYC2, reactivity in a coupled assay with ent-CDP synthase, EC 5.5.1.13, overview Kitasatospora griseola ?
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 ?
terpentedienyl diphosphate
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 Kitasatospora griseola terpentetriene + diphosphate
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 ?
terpentedienyl diphosphate generation of terpentetriene from through a diphosphate-removal reaction Kitasatospora griseola terpentetriene + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
Cyc2
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 Kitasatospora griseola
diterpene cyclase-2
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 Kitasatospora griseola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Kitasatospora griseola
50
-
-
 Kitasatospora griseola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Kitasatospora griseola
7.5
-
 assay at Kitasatospora griseola

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.5
-
 Kitasatospora griseola

General Information

General Information Comment Organism
additional information the CYC2 enzyme involves a DDXXD motif in the sequence that is characteristic to type-A cyclase and responsible for the diphosphate-removal reaction through Mg2+ chelation Kitasatospora griseola