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Literature summary for 4.2.3.32 extracted from

  • Keeling, C.I.; Weisshaar, S.; Lin, r.P.C.; Bohlmann, J.
    Functional plasticity of paralogous diterpene synthases involved in conifer defense (2008), Proc. Natl. Acad. Sci. USA, 105, 1085-1090.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
homology model of the second active site of enzyme based on the structure of 5-epiaristolochene synthase from Nicotiana tabacum, Protein Data Bank ID code 5EAT Picea abies

Protein Variants

Protein Variants Comment Organism
A713S products are isopimaradiene and sandaracopimaradiene Picea abies
G651V no change in product Picea abies
additional information swapping of residues 568-640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes Picea abies
V717L no change in product Picea abies
W679L/Y686H/A713S/V717L products are isopimaradiene and sandaracopimaradiene Picea abies
Y686H no change in product Picea abies
Y686H/A713S main products are isopimaradiene and sandaracopimaradiene Picea abies
Y686H/A713S A713S main products are isopimaradiene and sandaracopimaradiene Picea abies
Y686H/A713S/V717L products are isopimaradiene and sandaracopimaradiene Picea abies

Organism

Organism UniProt Comment Textmining
Picea abies Q675L4 bifunctional levopimaradiene/abietadiene synthase
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-copalyl-diphosphate reaction intermediate is C8-sandaracopimaradienyl cation Picea abies (-)-abietadiene + levopimaradiene + neoabietadiene + palustradiene + diphosphate bifunctional levopimaradiene/abietadiene synthase produces a mixture of diterpenes ?