Crystallization (Comment) | Organism |
---|---|
homology model of the second active site of enzyme based on the structure of 5-epiaristolochene synthase from Nicotiana tabacum, Protein Data Bank ID code 5EAT | Picea abies |
Protein Variants | Comment | Organism |
---|---|---|
A713S | products are isopimaradiene and sandaracopimaradiene | Picea abies |
G651V | no change in product | Picea abies |
additional information | swapping of residues 568-640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes | Picea abies |
V717L | no change in product | Picea abies |
W679L/Y686H/A713S/V717L | products are isopimaradiene and sandaracopimaradiene | Picea abies |
Y686H | no change in product | Picea abies |
Y686H/A713S | main products are isopimaradiene and sandaracopimaradiene | Picea abies |
Y686H/A713S A713S | main products are isopimaradiene and sandaracopimaradiene | Picea abies |
Y686H/A713S/V717L | products are isopimaradiene and sandaracopimaradiene | Picea abies |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | Q675L4 | bifunctional levopimaradiene/abietadiene synthase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl-diphosphate | reaction intermediate is C8-sandaracopimaradienyl cation | Picea abies | (-)-abietadiene + levopimaradiene + neoabietadiene + palustradiene + diphosphate | bifunctional levopimaradiene/abietadiene synthase produces a mixture of diterpenes | ? |