Cloned (Comment) | Organism |
---|---|
gene KSL5, sequence comparisons and phylogenetic analysis and tree | Oryza rufipogon |
gene KSL5j, sequence comparisons and phylogenetic analysis and tree | Oryza sativa Japonica Group |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Oryza sativa Japonica Group | |
Mg2+ | required | Oryza rufipogon |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Oryza sativa Japonica Group | - |
ent-pimara-8(14),15-diene + diphosphate | - |
? | |
ent-copalyl diphosphate | Oryza rufipogon | - |
ent-pimara-8(14),15-diene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza rufipogon | LC169117 | cv. W0106 | - |
Oryza sativa Japonica Group | Q6Z5J6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa Japonica Group | - |
leaf | - |
Oryza rufipogon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Oryza sativa Japonica Group | ent-pimara-8(14),15-diene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Oryza rufipogon | ent-pimara-8(14),15-diene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ent-kaurene synthase-like 5 | UniProt | Oryza sativa Japonica Group |
KSL5 | - |
Oryza sativa Japonica Group |
KSL5 | - |
Oryza rufipogon |
OrKSL5 | - |
Oryza rufipogon |
OsKSL5j | - |
Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
evolution | enzymes KSLs belong to the type A/class I terpene synthase family | Oryza rufipogon |
evolution | enzymes KSLs belong to the type A/class I terpene synthase family. The KSL5 members are clearly divided into two clades, representing japonica and indica groups. The KS responsible for gibberellin biosynthesis in a putative common ancestor, and OsKS1, which produces the tetracyclic ent-kaurene, has Ile as the corresponding key amino acid. Thus, it is suggested that substitution from Ile664 to Thr664 in KSL5 occurred before the onset of cultivation in the putative japonica ancestor. The OsKSL5 sequence is similar to the OsKSL6 sequence (89% identity), and it is therefore likely that the two KSL genes were generated through relatively recent duplication. The substitution in KSL5 probably occurred after duplication of the ancestral gene | Oryza sativa Japonica Group |
physiological function | recombinant OrKSL5 converts ent-CDP to ent-pimara-8(14),15-diene (EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-CDP to ent-isokaurene (EC 4.2.3.103). The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively, as is the case for Oryza sativa OsKSL5 and OsKSL5ind | Oryza rufipogon |
physiological function | the KSL5 members are clearly divided into two clades, representing japonica and indica groups, the 664th amino acids of OsKSL5j and OsKSL5i are Thr and Ile leading to different activity and production of ent-isokeurene synthase (EC 4.2.3.103) or ent-pimara-8(14),15-diene (EC 4.2.3.30) | Oryza sativa Japonica Group |