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Literature summary for 4.2.3.30 extracted from

  • Toyomasu, T.; Miyamoto, K.; Shenton, M.R.; Sakai, A.; Sugawara, C.; Horie, K.; Kawaide, H.; Hasegawa, M.; Chuba, M.; Mitsuhashi, W.; Yamane, H.; Kurata, N.; Okada, K.
    Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon (2016), Biochem. Biophys. Res. Commun., 480, 402-408 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene KSL5, sequence comparisons and phylogenetic analysis and tree Oryza rufipogon
gene KSL5j, sequence comparisons and phylogenetic analysis and tree Oryza sativa Japonica Group

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryza sativa Japonica Group
Mg2+ required Oryza rufipogon

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Oryza sativa Japonica Group
-
ent-pimara-8(14),15-diene + diphosphate
-
?
ent-copalyl diphosphate Oryza rufipogon
-
ent-pimara-8(14),15-diene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza rufipogon LC169117 cv. W0106
-
Oryza sativa Japonica Group Q6Z5J6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza sativa Japonica Group
-
leaf
-
Oryza rufipogon
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
Oryza sativa Japonica Group ent-pimara-8(14),15-diene + diphosphate
-
?
ent-copalyl diphosphate
-
Oryza rufipogon ent-pimara-8(14),15-diene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
ent-kaurene synthase-like 5 UniProt Oryza sativa Japonica Group
KSL5
-
Oryza sativa Japonica Group
KSL5
-
Oryza rufipogon
OrKSL5
-
Oryza rufipogon
OsKSL5j
-
Oryza sativa Japonica Group

General Information

General Information Comment Organism
evolution enzymes KSLs belong to the type A/class I terpene synthase family Oryza rufipogon
evolution enzymes KSLs belong to the type A/class I terpene synthase family. The KSL5 members are clearly divided into two clades, representing japonica and indica groups. The KS responsible for gibberellin biosynthesis in a putative common ancestor, and OsKS1, which produces the tetracyclic ent-kaurene, has Ile as the corresponding key amino acid. Thus, it is suggested that substitution from Ile664 to Thr664 in KSL5 occurred before the onset of cultivation in the putative japonica ancestor. The OsKSL5 sequence is similar to the OsKSL6 sequence (89% identity), and it is therefore likely that the two KSL genes were generated through relatively recent duplication. The substitution in KSL5 probably occurred after duplication of the ancestral gene Oryza sativa Japonica Group
physiological function recombinant OrKSL5 converts ent-CDP to ent-pimara-8(14),15-diene (EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-CDP to ent-isokaurene (EC 4.2.3.103). The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively, as is the case for Oryza sativa OsKSL5 and OsKSL5ind Oryza rufipogon
physiological function the KSL5 members are clearly divided into two clades, representing japonica and indica groups, the 664th amino acids of OsKSL5j and OsKSL5i are Thr and Ile leading to different activity and production of ent-isokeurene synthase (EC 4.2.3.103) or ent-pimara-8(14),15-diene (EC 4.2.3.30) Oryza sativa Japonica Group